带对准光路的激光细胞穿孔显微物镜设计  

Design of Microscopic Objective for Laser Cell Perforation with an Aligned Optical Path

作　　者：黄幼萍 林峰[1] 

机构地区：[1]福建师范大学光电与信息工程学院医学光电科学与技术教育部重点实验室,福建福州350007

出　　处：《红外技术》2015年第4期305-310,共6页Infrared Technology

基　　金：福建省科技厅重点项目;编号:2013H0022;福州市科技计划项目;编号:2014-G-68

摘　　要：针对近红外光谱可对活体组织进行无损伤穿孔，并解决在穿孔中需要重新对焦的问题，通过Zemax软件对7片式前置光阑显微物镜结构在0.48～1.48mm波长范围内进行了宽光谱消色差的优化设计。设计实现了像距为无限远的多光谱共焦成像，其放大倍率为40，数值孔径0.5，最大焦面漂移量仅为27mm，可见光波段在空间频率为350 lp/mm时，0.7视场的MTF＞0.5，具有较高的分辨力。并且在波长1480 nm处，MTF值接近衍射极限，具有较高的能量，满足细胞穿孔的要求。文中还给出了实验结果，并预测其应用前景。Since living tissue can be perforated without injury bynear infrared spectrum, and in order to solve the problem of refocusing, the 7 pieces of front aperture microscope structure is designed and optimized in the 0.48-1.48mm waveband by using Zemax software. It realizes apochromatism and multi-spectral confocal imaging with the image distance of infinity, and the maximum focal shift is only 27mm. At the same time, the optical magnification reaches 40 and number aperture is 0.5. The Optimal Structure Designing shows that the system has high resolution, the MTF of visible wavelengths beyond 0.5 at a spatial frequency of 350lp/mm in 0.7 field. In addition, the MTF of 1480nm wavelength is close to the diffraction limit and the energy is high, which caters for the requirements of cell perforation. In the meantime, the experimental results are drawn out and we have made predictions about its prospects.

关 键 词：消色差显微物镜 激光细胞穿孔 多光谱共焦成像 Zemax软件 无限远像距 

分 类 号：TH773[机械工程—仪器科学与技术]