红柄白鹃梅叶中黄酮类物质的提取研究  

Research of Extracting Flavonoids from Ecochorda giraldii

作　　者：邓荣华[1] 王颖[1] 王慧[1] 郭晓宏[1] 魏学智[1] 

机构地区：[1]山西师范大学生命科学学院,山西临汾041004

出　　处：《中国野生植物资源》2015年第2期5-8,共4页Chinese Wild Plant Resources

基　　金：国家自然科学基金(30972396;31300157);山西省自然科学基金项目资助(2009011041-1)

摘　　要：采用乙醇提取,用分光光度法测定的方法,以红柄白鹃梅为原料,通过正交试验研究了乙醇浓度,提取温度,提取时间和料液比4个因素对提取红柄白鹃梅叶片中总黄酮含量的影响。研究结果表明,各试验因素对红柄白鹃梅叶中黄酮类物质提取量的影响依料液比、提取温度、乙醇浓度、提取时间因素顺次降低,当提取温度为70℃、提取时间为2 h、料液比为1∶20时,提取效果最好,黄酮类物质提取量为22.412 mg/g。In this paper, we used Ecochorda giraldii as raw material. The content of flavonoids in Eco- chorda giraldii was extracted by ethanol and determined by spectrophotometry. The orthogonal test was a- dopted to study the effects of four factors including ratio of material to liquid, extracting temperature, eth- anol concentration, extracting time on the extraction rate of flavonoids from Ecochorda giraldii leaves. The results showed that the processing conditions affected the yield, and that the effect of each condition decreased in the following order： ratio of material to liquid, extracting temperature, ethanol concentra- tion, extracting time. The optimum condition of extracting total flavonoids was with 70% ethanol, ratio of material to liquid of 1 ： 20, at 70℃, for 2 h, under which the total flavonoids yield could reach 22. 412 mg/g.

关 键 词：红柄白鹃梅 黄酮类 提取 

分 类 号：Q946.8[生物学—植物学] Q949.91