基于常压室温等离子体技术的金藻诱变筛选方法  

Mutagenic Screening Method of Isochrysis zhangjiangensis by Atmospheric and Room Temperature Plasmas

作　　者：曹旭鹏[1] 艾江宁[1] 刘亚男[1] 周建男[1] 吴佩春[1] 薛松[1] 

机构地区：[1]中国科学院大连化学物理研究所,大连116023

出　　处：《中国生物工程杂志》2014年第12期84-90,共7页China Biotechnology

基　　金：国家"863"计划(2012AA052101)资助项目

摘　　要：以高生长速率或高油脂含量藻株为目标,以湛江等鞭金藻为例,报道了一种基于常压室温等离子体技术的微藻诱变及快速分级筛选方法。即以叶绿素荧光动力学参数Fv/Fm大于0.68的金藻为出发藻株、以致死率90%为阈值确定最适诱变电流为1.4~1.5A,诱变时间为24~30s。分别在室温常光及胁迫条件（高温常光和高光室温）下,按照孔板至摇瓶至反应器三级培养进行筛选。在孔板培养过程中以比生长速度结合尼罗红荧光强度变化实现高通量初筛,最终在反应器培养中进行提取验证,并以候选藻株为出发进行二次诱变筛选。结果表明,室温常光条件诱变株筛出率为0.7%,胁迫条件筛出率为0.9%;一次诱变诱变株筛出率为0.6%,二次诱变筛出率为1.2%;二次诱变和胁迫条件筛出率更高,更易得到性状变化的诱变株。To select algal strains with fast growth rate or high oil content,Isochrysis zhangjiangensis is mutated with atmospheric and room temperature plasmas（ ARTP）. The mutants are screened by well and flask at room temperature ordinary light conditions and stress conditions,such as high temperature ordinary light,high light ordinary temperature. An integrated screening method is set up. Through 600 ml bioreactor cultivated evaluation,the rate of the detection of mutants is 0. 7% at room temperature ordinary light conditions,1. 1% at stress conditions,0. 6% through once mutagenesis and 1. 2% through secondary mutagenesis. The results show that screening rate is high at secondary mutagenesis and stress conditions. It is easier to obtain mutants with character change.

关 键 词：湛江等鞭金藻 诱变藻株 常压室温等离子体诱变 胁迫条件 二次诱变 

分 类 号：Q819[生物学—生物工程]