基于高内涵分析的何首乌对肝窦内皮细胞损伤的配伍减毒研究  

Compatibility Attenuated Detoxification study on Radix Polygoni Multiflori-caused Hepatic Sinus Endothelial Cell Injury Based on High Content Analysis

作　　者：庞晶瑶 李雨萌[3] 柏兆方[2] 赵艳玲[2] 赵奎君[1] 马致洁[1] 王伽伯[2] 肖小河[2] 

机构地区：[1]首都医科大学附属北京友谊医院,北京100050 [2]解放军302医院全军中药研究所,北京100039 [3]云南中医学院中药学院,云南昆明650500

出　　处：《中国现代中药》2015年第4期331-334,共4页Modern Chinese Medicine

基　　金：国家重大新药创制专项课题(2015ZX09501-004-001-008);国家公益性行业专项课题(201507004-04);国家自然科学基金项目(81373984;81403126)

摘　　要：目的:观察何首乌对肝窦内皮细胞损伤的配伍减毒情况,为其临床合理用药提供参考。方法:采用高内涵分析技术,检测何首乌与茯苓、甘草、三七在肝窦内皮细胞上的配伍减毒情况。结果:何首乌对肝窦内皮细胞的损伤呈剂量依赖性,在IC50的剂量下,分别配伍不同浓度的茯苓、甘草、三七都有显著的减毒作用,且呈剂量依赖性。何首乌配伍茯苓减毒的效果较好,甘草次之,三七稍差。结论:采用高内涵分析方法更加直观、具体地反映何首乌与茯苓、甘草、三七的配伍减毒效果,为临床用药的安全性、合理性提供依据。Objective： To observe the compatibility attenuated detoxification of Radix Polygoni Muhiflori-caused hepatic sinus endothelial cell injury, and provide reference for the further study of the clinical rational medication. Methods： The High Content Analysis was applied to detect the compatibility attenuated detoxification of Radix Polygoni Multiflori-caused hepatic sinus endothelial cell injury with Poria cocos, Radix Glycyrrhizae and Radix notoginseng. Result： A dose dependent relation existed between Radix Polygoni Multiflori and hepatic sinus endothelial cells injury, which was embedded significantly in compatibility attenuated detoxification with different concentration of Poria cocos, Radix Glycyrrhizae and Radix notoginsen under the dose of IC50. Compatibility attenuated detoxification with Poria cocos effected better, of which with Radix notoginseng was the least potent. Conclusion： Compatibility attenuated detoxification effect of Radix Polygoni Muhiflori and Poria cocos, Radix Glycyrrhizae and Radix notoginsen was specifically reflected through High Content Analysis, which may provide a scientific basis for the safety and rationality of clinical medication.

关 键 词：何首乌 配伍减毒 肝窦内皮细胞 高内涵分析 

分 类 号：R657.3[医药卫生—外科学]