激光多普勒对糖尿病小鼠微循环自律运动功能的评估  

Evaluation of Microvascular Vasomotion in STZ-Induced Diabetic Mice by Laser Doppler

作　　者：刘明明[1] 张晓艳[1] 李炳蔚[1] 王冰[1] 刘淑英[1] 盛有明[1] 李宏伟[1] 修瑞娟[1] 

机构地区：[1]中国医学科学院北京协和医学院微循环研究所卫生部微循环重点实验室,北京100005

出　　处：《现代生物医学进展》2015年第13期2408-2412,共5页Progress in Modern Biomedicine

基　　金：协和青年基金和中央高校基本科研业务费专项资金(33320140061)

摘　　要：目的:探讨激光多普勒对糖尿病小鼠微循环自律运动功能的评估价值。方法:20只BALB/c小鼠随机分为糖尿病组(n=10)和对照组(n=10)。连续5天腹腔注射40 mg/kg STZ诱导糖尿病模型;应用Moor LDLS检测糖尿病小鼠皮肤微循环血流灌注水平;应用Moor VMS-LDF检测糖尿病小鼠胰腺微循环血流灌注及微血管自律运动功能。结果:糖尿病小鼠皮肤微循环血流灌注显著降低,皮肤总血流灌注量(P=0.009)、胰腺微血管自律运动频率(P<0.001)和振幅(P<0.001)均显著低于对照组小鼠。结论:联合应用Moor LDLS激光多普勒扫描成像系统及Moor VMS-LDF激光多普勒血流灌注监测系统可作为糖尿病小鼠微循环功能状态的评估手段。Objective： To evaluate the application of laser doppler in assessing microvascular vasomotion of STZ-induced diabetic mice. Methods： 20 BALB/c mice were randomly divided into diabetic group （n=10） and control group （n=10）. Diabetic mice were in- duced with 40 mg/kg STZ for 5 consecutive days. Cutaneous blood perfusion was determined by Moor LDLS, while pancreatic blood perfusion and micro-vessels vasomotion were detected by Moor VMS. Results： Compared with control group, the cutaneous blood perfusion and total flux （P=0.009） was decreased, and the frequency and amplitude of vasomotion of pancreatic micro-vessels was also decreased in diabetic mice （P〈0.001, P〈0.001, respectively）. Conclusion： Combined Moor LDLS and Moor VMS-LDF might be an applicable method to evaluate microvascular vasomotion of diabetic mice.

关 键 词：激光多普勒 微循环 糖尿病 

分 类 号：Q95-3[生物学—动物学] R587.1[医药卫生—内分泌]