七氟烷对培养的小鼠小胶质细胞炎症因子表达的影响（英文）  

Effect of Sevoflurane on the Expression of Inflammation Cytokine in Microglia of Cultured Mice

作　　者：孙敏[1] 张磊[2] 宋淑玲[3] 陈作雷[4] 刘英志[4] 殷积慧[4] 

机构地区：[1]青岛大学医学院,山东青岛266071 [2]上海交通大学医学院附属第九人民医院,上海200011 [3]滨州市中心医院,山东滨州251700 [4]青岛大学附属医院,山东青岛266000

出　　处：《现代生物医学进展》2015年第15期2840-2844,共5页Progress in Modern Biomedicine

基　　金：The National Natural Science Foundation of China(81300934)~~

摘　　要：目的：探讨七氟烷对培养的小鼠小胶质细胞中炎症因子表达的影响。方法：取新生（2~3天）C57BL/6小鼠,分离小胶质细胞,将其随机分为4组（n=10）：对照组（Control）;七氟烷组（Sevoflurane）;NF-κB抑制剂组（PDTC）;NF-κB抑制剂＋七氟烷组（PDTC＋Sevoflurane）。用Drager麻醉机向Sevoflurane组PDTC＋Sevoflurane组培养的小胶质细胞盒内释放21%O2,5%CO2,4.1%七氟烷的气体,用气体分析仪持续监测各组的浓度。应用Iba-1的免疫荧光染色法对小鼠小胶质细胞进行纯度鉴定。分别在于给七氟烷后2 h、4 h和6 h时采用免疫印迹分析技术检测两组小胶质细胞IL-6和TNF-α的表达水平和NF-κB的活性。PDTC＋Sevoflurane组在给七氟烷前一小时给予PDTC,采用ELISA技术和免疫印迹分析技术检测各组小胶质细胞IL-6和TNF-α的浓度和NF-κB的表达。结果：免疫印迹显示七氟烷组细胞中IL-6、TNF-α水平和NF-κB的激活水平升高;PDTC降低了七氟烷作用后核内NF-κB的表达,减弱了IL-6和TNF-α水平的升高作用。结论：七氟烷可通过激活NF-κB信号通路,进一步激活培养的小鼠小胶质细胞中炎症因子的表达。Objective： To evaluate effect of sevoflurane on the expression of inflammation cytokine in neuroglioma cells of cul- tured mice. Methods： Microglia cells abtained fiom newborn （2-3 days） C57BL/6 mice were separated and randomly divided into 4 groups （n=10）： control group （Control）; sevoflurane group （Sevoflurane）; NF-KB inhibitors group （PDTC）; NF-KB inhibitors group＋ sevoflurane group （PDTC＋Sevoflurane）. Oxygen （21%）, Carbon dioxide （5 %） and sevoflurane（4.1%） were delivered from an Drager anesthesia machine to the microglia cells sealed in a plastic box of the two groups. The two groups are Sevoflumne group and PDTC＋Sevoflumne group in which the later group had been given PDTC 1 hour before sevoflurane treatment. A Drag gas analyzer was used to continuously monitor the concentrations of the 02, CO2 and sevoflurane respectively. Iba-1 immunofluorescence staining method was used to the purity identification of microglia in mice. We examined the expression of IL-6 and TNF-a （by Western Blotting Analysis and ELISA）and activation of NF-KB （by Western Blotting Analysis） at 2,4 and 6h after sevoflurane treatment. Results： Sevoflurane increases levels of IL-6 and TNF-a through activates NF-kB signaling pathway. Conclusion： These studies suggest that NF-kB signaling pathway could contribute to the increasing expression of inflammation cytokine.

关 键 词：七氟烷 神经炎症 IL-6 TNF-α NF-ΚB 

分 类 号：R164.1[医药卫生—公共卫生与预防医学]