RICH2在肝细胞肝癌中蛋白表达的研究  

Study on the Protein Expression of RICH2 in Hepatocelular Carcinoma

作　　者：张佳瑞[1] 巩丽[1] 杨晨[2] 朱少君[1] 韩秀娟[1] 姚丽[1] 王姝妹[1] 张伟[1] 李艳红 

机构地区：[1]第四军医大学唐都医院病理科,陕西西安710038 [2]第四军医大学唐都医院神经外科,陕西西安710038 [3]第四军医大学唐都医院妇产科,陕西西安710038

出　　处：《现代生物医学进展》2015年第15期2848-2850,共3页Progress in Modern Biomedicine

基　　金：国家自然科学基金项目(30672013;81372226);陕西省科技统筹创新工程(2011KTCL03-11)

摘　　要：目的:分析肝细胞肝癌与正常肝组织中RICH2蛋白表达之间的关系,探讨RICH2在肝细胞肝癌发生发展中的作用机理。方法:选取19例肝细胞肝癌患者作为研究对象,用免疫组织化学方法检测肝癌组织和癌旁正常组织中RICH2的表达情况并采用Friedrich免疫反应评分法作为判定标准。采用非参数Wilcoxon秩和检验对RICH2在肝细胞肝癌和正常组织中的评分结果进行统计学分析。结果:19例HCC病例癌旁正常组织中RICH2表达阳性率为100%(19/19),其中RICH2表达强阳性(+++)5例(26.3%),RICH2表达弱阳性(+)和中阳性(++)分别为3例(15.8%)和11例(57.9%);而HCC组织除了两例肝硬化组织未取到癌组织外,17例病例中RICH2表达阳性率仅为11.8%(2/17),表达为+和++各占50%。肝癌组织中RICH2的表达水平显著低于癌旁组织。结论:RICH2有可能通过某种或者其中几种因素作用失活,作用于信号通路,进而促进肝细胞肝癌的发生发展。研究其作用机理,可为临床诊断、治疗及预后评估提供理论依据。Objective： To analyze RICH2 expression levels of hepatocellular carcinoma and normal paraneoplastic tissue, and to investigate the role of RICH2 in the occurrence and development of HCC. Methods： Immunohistoehemieal method was used to detect the expression levels of Rich2 in 19 cases of HCC tissues and their adjacent tissues. Friedrieh immunoreaetivity score was used as the judge criteria and the grades of HCC and normal tissue were statistical analyzed according to Wilcoxon Nonparam etrierank-sum test. Results： I00 percent of normal tissue in the HCC eases have positive RICI-I2 expression （19/19）, of which 5 cases expressed positive RICH2 strongly （＋＋＋）（5cases,26.3 %）, 3 cases expressed positive RICH2 weakly （＋）（3eases, 15.8 %） and I leases expressed positive RICH2 moderately（＋＋）（1 leases, 57.9 %）. While the percentage of positive RICH2 of 17 HCC cases was 11.8 %（2/17）, case scored ＋ and ＋＋ac-counts a half respectively. Carcinoma tissue were failed to be got of the other 2 cases because the specimens only showed hepatic cirrhosis. The result showed a lower RICH2 expression in the HCC tissue than the normal tissue. Conclusions： Inactivation of RICH2 resulted from certain factors affects the signal pathway in advance induces the HCC. We aim to research the pathogenesis to assist on the clinic diagnosis, treatment and prognosis.

关 键 词：肝细胞肝癌 免疫组织化学 Friedrich免疫反应评分法 RICH2 

分 类 号：R512.62[医药卫生—内科学]