家蝇抗菌肽对FBL-3红白血病原代细胞增殖影响浓度的探索  

EFFECT OF THE CONCENTRATION OF MUSCA DOMESTICA ANTIMICROBIAL PEPTIDE ON THE PROLIFERATION OF PRIMARY FBL-3 CELLS

作　　者：杜斌[1] 赵瑞君[1] 程璟侠[2] 王文健[1] 原发家[1] 聂守民 李彦红[3] 

机构地区：[1]山西医科大学基础医学部,寄生虫学教研室,太原030001 [2]山西省疾病预防控制中心病媒生物防控科,太原030001 [3]山西医科大学附属第二医院心内科,太原030001

出　　处：《寄生虫与医学昆虫学报》2015年第1期24-28,共5页Acta Parasitologica et Medica Entomologica Sinica

基　　金：山西省基础研究计划（自然科学基金）项目（2013011060-4）;太原市科学技术发展计划项目（081049）;山西医科大学科技创新基金（01200908）

摘　　要：本文初步探究不同浓度家蝇幼虫抗菌肽对FBL-3红白血病原代细胞增殖的影响。采用诱导、研磨、离心、C18固相萃取等方法制备家蝇幼虫抗菌肽混合物，然后处理传代期的FBL-3细胞，调整浓度为2．25×10^6／mL并通过尾静脉注射C57BL／6小鼠，建立红白血病动物模型。待肿瘤生长较大时，处死并剥离肿瘤，再通过研磨、裂红液裂解、获取原代肿瘤细胞并培养。将抗菌肽混合物调整为不同浓度作用于传代期的FBL-3原代细胞，之后经倒置显微镜观察照相，台盼蓝染色试验计算死亡率、CCK-8试剂盒做细胞毒性检测。结果显示抗菌肽混合物浓度为250—350μg／mL作用FBL-3原代细胞时，细胞碎片大量增多，死亡率增高，细胞毒性变大。初步证明了抗菌肽混合物在250～350μg／mL抑制细胞增殖效果明显，在300μg／mL浓度下抑制作用最强，本研究结果可作为动物模型体内实验的参考。The inhibition effect of antimicrobial peptides from the housefly （Musca domestica） larvae on primarycell FBL- 3 was tested under laboratory. Antibacterial peptides extracts was purified with C18 solid phase methods from induced housefly larva. Erythroleukemia mice model was prepared by injected 2.25 × 10^6/ mL FBL-3 ceils into C57BL/6 mouse via the tail vein. The primary culture of FBL-3 cells was harvested from the mice and utilized to test the inhibition effect of the antibacterial peptide extracts. When observed with trypan blue staining methods, antibacterial peptide mixture at a concentration of 250 - 350μg/mL affects FBL-3 primary cells significantly with increased cell debris and more mortality rate. Cell apoptosis was also shown in the concentration range tested with CCK-8 kit. The reference concentration of antibacterial peptide would be applied in further tests in vivo.

关 键 词：家蝇 抗菌肽 FBL-3原代细胞 浓度 

分 类 号：Q2-33[生物学—细胞生物学]