黑龙江口岸斑点热群立克次体调查研究  

INVESTIGATION OF SPOTTED FEVER GROUP RICKETTSIA IN HEILONGJIANG PORT

作　　者：鞠文东 程成 付维明 王延禄 呼满霞[2] 梁慧杰 王红霞 徐宁 耿聪 

机构地区：[1]黑龙江国际旅行卫生保健中心,哈尔滨150001 [2]绥芬河出入境检验检疫局,黑龙江绥芬河157301 [3]萝北出入境检验检疫局,黑龙江萝北154201 [4]同江出入境检验检疫局,黑龙江同江156401

出　　处：《寄生虫与医学昆虫学报》2015年第1期37-43,共7页Acta Parasitologica et Medica Entomologica Sinica

基　　金：国家质量监督检验检疫总局科研基金（2014IK047）;黑龙江出入境检验检疫局科研基金（2013HK006）

摘　　要：为了解黑龙江口岸地区斑点热群立克次体在人群、鼠类、蜱类中的分布特征，针对黑龙江省9个中俄边境口岸，采集出入境人群的血液样本215份、鼠类样本204份、蜱类样本188份，利用半巢式PCR方法对所有样本进行斑点热群立克次体特异性片段扩增，通过基因测序对斑点热群立克次体分型。结果在哈尔滨机场及嘉荫两个口岸的大仓鼠及小家鼠中检测到2例劳氏立克次体Rickettsia raoultii，为首次在黑龙江口岸鼠样本中检测出劳氏立克次体；在188份蜱样本中，检出斑点热群立克次体阳性样本101例，感染率达53．7％，其中劳氏立克次体89例，新塔拉塞维奇立克次体Candidatus Rickettsia tarasevichiae11例，黑龙江立克次体Rickettsia heilottgjiaagii1例，同样首次在黑龙江口岸全沟硬蜱中检测到新塔拉塞维奇立克次体。黑龙江中俄边境口岸开展SFGR的长期监测具有非常重要的公共卫生学意义。To understand the distribution characteristics of spotted fever group Rickettsia in the residencies, rodents and ticks in Heilongjiang ports, total 9 port area along with Sino-Russian border were sampled to test the species and infection rates of spotted fever group Rickettsia from 188 ticks and 204 rodents. Moreover, 215 humans entry or exit from these ports were also include for screening the possible infection the rickettsia organisms under their permissions. Results from seminested PCR and sequences indicated that two Rickettsia raoultii detected in Cricetulus triton and Mus musculus Linnaeus respectively. None rickettsia organism were found in the human samples. While in ticks, 90 R. raoultii, 11 Candidatus Rickettsia tarasevichiae and 1 R. heilongiangii were found. The preliminary suggested the risk of emerged spotted fever pathogens in these areas and the necessary and urgent of long term monitoring on tick borne diseases.

关 键 词：斑点热群立克次体 基因测序 劳氏立克次体 新塔拉塞维奇立克次体 

分 类 号：Q939.3[生物学—微生物学]