烟草PHOT2基因cDNA片段的克隆与RNAi表达载体的构建  

Cloning c DNA Fragment of PHOT2 Gene of Tobacco and Construction of Its RNAi Vector

作　　者：乔新荣[1] 陈琼[1] 郭桥燕[2] 徐长水[3] 

机构地区：[1]信阳农林学院,河南信阳464000 [2]河南省烟草公司平顶山市公司,河南平顶山467000 [3]河南大学生命科学学院/植物逆境生物学重点实验室,河南开封475004

出　　处：《湖北农业科学》2015年第4期981-983,987,共4页Hubei Agricultural Sciences

摘　　要：采用生物信息学方法,以番茄(Solanum lycopersicum)PHOT2基因序列为探针,比对了烟草(Nicotiana tabacum)EST数据库,并根据获得的同源性较高的EST序列设计引物,PCR扩增了烟草K326PHOT2基因的c DNA片段。结果表明,成功扩增出了PHOT2基因的c DNA片段,并构建了干扰烟草PHOT2基因表达的RNAi表达载体。Using coding sequence of tomato （Solarium lycopersicum） PHOT2 gene as probe, EST database of Tobacco （Nico- tiana tabacum） were searched and compared. Primers were designed with high homologous sequence obtained. The cDNA fragments of PHOT2 gene of tobacco K326 were amplied with PCR. The results showed that the eDNA fragments of PHOT2 gene were successfully amplied. RNAi expression vector of tobacco PHOT2 gene was constructed.

关 键 词：烟草(Nicotiana tabacum) 向光素 克隆 载体 

分 类 号：Q781[生物学—分子生物学]