银杏黄酮对非酒精性脂肪肝小鼠肝脏Toll样受体-4和核因子-κB表达的影响  

Effects of ginkgo flavone on the expression of TLR4 and NF-κB in the liver of mice with nonalcoholic fatty liver disease

作　　者：白纪红[1] 梁志清[1] 赵日红[1] 刘艳华[1] 林秋香[1] 赵雪[1] 

机构地区：[1]桂林医学院附属医院,541001

出　　处：《实用医学杂志》2015年第7期1091-1095,共5页The Journal of Practical Medicine

基　　金：国家自然科学基金项目(编号:81260078);广西自然科学基金项目(编号:2012GXNSFAA276038;2014GXNSFAA118152)

摘　　要：目的:观察银杏黄酮对非酒精性脂肪肝(NAFLD)模型小鼠肝脏Toll样受体-4(TLR4)和核因子-κB(NF-κB)表达的影响。方法:120只昆明小鼠随机分为对照组、模型组和银杏黄酮高、中、低剂量组。采用高脂饲料构建NAFLD小鼠动物模型,于给药后8周末处死,观察肝脏病理变化,计算肝指数,检测血清肿瘤坏死因子-α(TNF-α)、白介素-6(IL-6)、甘油三酯(TG)以及肝组织NF-κB和TLR4的表达。结果:银杏黄酮组小鼠肝指数和血清TG、TNF-α、IL-6水平及肝组织NF-κBp65的表达量比模型组显著降低,肝脏脂肪变性明显减轻,但肝组织TLR4表达差异无统计学意义(P>0.05)。结论:TLR4/NF-κB炎症通路与NAFLD的发病有关,银杏黄酮可改善肝组织病理变化,起到抗炎降脂作用,从而对NAFLD起到一定的治疗作用,但对TLR4影响并不明显。Objective To investigate the effects of ginkgo flavone on the expression of NF-~B and TLR4 in the liver of mice with nonalcoholic fatty liver disease （NAFLD）. Methods 120 KM mice were randomly divided into the control group, model group, as well as high, medium and low dosage of ginkgo flavone groups. The animal model of NAFLD in mice was constructed with high fat diet. The pathological changes of liver, liver index, the serum TNF-α, IL-6, TG, NF-KB and TLR4 in hepatic tissue was observed after 8 weeks of administration. Results Compared with the model group, the level of liver index, serum TG, TNF-a, IL-6 and the expression of NF-KBp65 in the ginkgo flavone groups dramatically decreased 8 weeks after the administration. And the hepatic steatosis was milder. There was no statistical differences in the expression of TLR4 between the ginkgo flavone groups and the control group （P 〉 0.05）. Conclusions These results suggested the closely relationship between TLR4/NF-KB inflammatory pathway and NAFLD. Ginkgo flavone had the therapeutical effects on NAFLD by anti-inflammatory and lipid-lowering action, but no effect was observed on the expression of TLR4 in hepatic tissue.

关 键 词：银杏黄酮 非酒精性脂肪肝病 TOLL样受体-4 核因子-ΚB 小鼠 

分 类 号：R285.5[医药卫生—中药学]