姜黄素对实验性关节炎大鼠关节滑液中VEGF及血清TNF-α表达的影响  

Effect of Curcumin on expression of VEGF in synovial fluid and TNF-α in the serum of Experimental Arthritis in rats

在线阅读下载全文

作  者:马意 陈美周 张鹏程[1] 余方流[1] 

机构地区:[1]皖南医学院,安徽芜湖241001

出  处:《中医药临床杂志》2015年第2期245-247,共3页Clinical Journal of Traditional Chinese Medicine

基  金:2013年度皖南医学院大学生科研基金(WK2013S09);安徽省高校省级自然科学研究项目(KJ2011B197)

摘  要:目的:观察姜黄素对实验性关节炎大鼠关节滑液中VEGF及血清TNF-α表达的影响,并初步探讨姜黄素对实验性关节炎大鼠治疗作用的可能机理。方法:取雄性SD大鼠,用弗氏完全佐剂(FCA)诱导产生实验性关节炎大鼠模型,将模型大鼠随机分组,即姜黄素组1(10.0g/kg)和姜黄素组2(20.0g/kg)等实验组及对照组,并对模型大鼠灌胃用药或生理盐水5周,ELISA法检测给药后模型大鼠血清中TNF-α及后膝关节腔滑液中VEGF的表达。结果:1用药5周后,关节炎症状明显缓解(AI积分减小),实验组大鼠体重均有所增长,以姜黄素组2尤为显著(P<0.01);2用药5周后,实验组大鼠血清中TNF-α及关节腔滑液中VEGF表达均明显降低,以姜黄素组2尤为显著(P<0.01)。结论:姜黄素对实验性关节炎大鼠有较好疗效,其治疗机制可能与抑制TNF-α与VEGF的表达有关。Objective: To observe the effect of curcumin on expression of VEGF in synovial fluidand and TNF- α in the serum of experimental arthritis in rats, and to investigate the possible mechanism of curcumin on therapeutic in experimental arthritis rats. Methods: some male SD rats with complete Freund' s adjuvant (FCA) induced model of experimental arthritis rats, the model rats were randomized into four groups, namely curcumin group 1 (10.0g/kg) and cureumin group 2 (20.0g/ kg) as experimental group and control group, and the model rats were medicine or physiology saline for 5 weeks, ELISA was used to detect the expression of VEGF in the cavity of the late knee joint synovial fluid and administration of TNF- α in model rat serum . Results: after 5 weeks, arthritis symptoms were relieved (the score of AI decreased), weight of each experimental group rats increased in eurcumin group, 2 particularly significant (P〈0.01); after 5 weeks of treatment, were significantly decreased the expression of VEGF in joint cavity synovial fluid and TNF-αt in serum of experimental rats, in curcumin group 2 is particularly significant (P〈O.O1). Conclusion: Curcumin has a better therapeutic on rat experimental arthritis and its therapeutic mechanism may be relevant to inhihiting the expression of TNF- α and VEGF.

关 键 词:类风湿性关节炎 姜黄素 血管内皮生长因子 TNF-Α 

分 类 号:R269[医药卫生—中西医结合]

 

参考文献:

正在载入数据...

 

二级参考文献:

正在载入数据...

 

耦合文献:

正在载入数据...

 

引证文献:

正在载入数据...

 

二级引证文献:

正在载入数据...

 

同被引文献:

正在载入数据...

 

相关期刊文献:

正在载入数据...

相关的主题
相关的作者对象
相关的机构对象