卵巢切除联合激素注射对绵羊股骨近端骨密度及生物力学性能的影响  

Effect of ovariectomy combined with hormone injection on bone density and biomechanical performance of sheep proximal femur

作　　者：刘达[1] 康夏[1] 谢庆云[1] 廖冬发[1] 黄晨[1] 唐影超 权毅[1] 张波[1] 

机构地区：[1]成都军区总医院骨科,四川成都610083

出　　处：《重庆医学》2015年第12期1606-1608,1612,共4页Chongqing medicine

摘　　要：目的评价去势手术联合激素注射对绵羊股骨近端骨密度(BD)及生物力学性能的影响。方法将健康成年绵羊16只,分为假手术组(n=8)和实验组(n=8)。假手术组仅对双侧卵巢进行显露;实验组中显露并切除双侧卵巢(OVX),术后1个月开始肌内注射泼尼松龙(0.45mg·kg-1·d-1),共持续注射10个月。测量处理前、后绵羊股骨近端的BD,通过压缩实验和轴向拔出实验评价处理后股骨近端的生物力学性能变化。结果处理前两组绵羊BD比较,差异无统计学意义(P>0.05);假手术组绵羊处理前、后股骨近端BD比较,差异无统计学意义(P>0.05);实验组绵羊处理后股骨近端的BD显著下降,且明显低于假手术组处理后的BD(P<0.05)。实验组的最大压缩应力和能量吸收值均显著低于假手术组,差异均有统计学意义(P<0.05);实验组的最大轴向拔除力和能量吸收值均显著低于假手术组,差异均有统计学意义(P<0.05)。结论去势手术联合激素注射的方法可以显著降低绵羊股骨近端的BD及生物力学强度。Objective To evaluate the effect of bilateral ovariectomy combined with hormone injection on the bone mineral density and biomechanical property of sheep proximal femur. Methods 16 healthy adult sheep were divided into the sham operation group （n=8） and the experiment group （n=8） randomly. Bilateral ovaries were only exposed in the sham operation group. The ex- periment group was performed bilateral ovariectomy （OVX） and began to conduct the intramuscular injection of methylprednisolone （0.45 mg · kg^-1·d^-1 ） after postoperative 1 month for 10 months. The bone density （BD） of all sheep proximal femur was meas- ured before OVX and in postoperative 1 year. The compression test and the axial pullout test were performed to evaluate biome- chanical property of postoperative 1 year proximal femur. Results BD of proximal femur before surgery had no statistically signifi- cant difference between the two groups, and which in the sham operation group had no statistically significant difference between before and after surgery （P〉0.05）. BD of proximal femur in postoperative 1 year in the experiment group was significantly de- creased and significantly lower than that in the sham operation group（P〈0.05）. The maximal compression stress and the energy absorption value in the experiment group were significantly lower than those in the sham operation group with statistically signifi- cant differences（P〈0.05） ;the maximal axial pulling force and the energy absorption value in the experiment group were signifi- cantly lower than those in the sham operation group with statistically significant difference（P〉0.05）. Conclusion The method of bilateral ovariectomy combined with hormone injection can significantly decrease BD and biomechanical intensity of sheep proximal femur.

关 键 词：骨质疏松 股骨近端 卵巢切除术 骨密度 生物力学 

分 类 号：R580[医药卫生—内分泌]