CHC患者外周血T细胞免疫功能与丙型肝炎病毒表达的关系  

Relationship between immune function of T cells in peripheral blood and expression of hepatitis C virus in patients with chronic hepatitis C

作　　者：栗群英[1] 梁明莉[1] 陈莉[1] 吴丽娟[1] 刘毓刚[1] 刘彤[1] 谢静[1] 胡宗海[1] 曲远青 

机构地区：[1]成都军区总医院实验医学中心/高湿医学全军重点实验室,四川成都610083

出　　处：《重庆医学》2015年第12期1620-1622,1625,共4页Chongqing medicine

基　　金：国家自然科学基金青年基金资助项目(81301445)

摘　　要：目的探讨慢性丙型肝炎(CHC)患者外周血T细胞免疫功能与丙型肝炎病毒(HCV)复制及核心抗原(HCV-cAg)表达的关系。方法采用流式细胞术、荧光定量PCR和ELISA对63例健康体检者(对照组)和85例CHC患者(CHC组)的外周血T淋巴细胞亚群、血清HCV-RNA和HCV-cAg进行测定。结果 CHC组总T细胞、T4细胞、T8细胞、双阴性细胞(DNT)、双阳性细胞(DPT)水平依次为(67.37±10.43)%、(37.11±10.28)%、(21.63±8.87)%、(7.80±4.57)%、(0.20±0.29)%,T4/T8比值为(2.18±1.26)%,绝对水平依次为(0.70±0.44)×109/L、(0.37±0.22)×109/L、(0.22±0.17)×109/L、(0.08±0.06)×109/L和(0.19±0.68)×107/L;CHC患者T8细胞水平显著减少(P<0.01),致使T4/T8比值升高(P<0.05);CHC患者总T细胞、T4细胞、T8细胞和DNT的绝对值均显著减少(P<0.05);HCV-RNA和HCV-cAg阳性时,T4细胞和DNT水平显著升高(P<0.05)。结论 CHC患者HCV复制表达时,患者T细胞亚群存在明显异常,T细胞免疫功能低下或免疫耐受是CHC迁延难愈的重要原因。Objective To explore the relationship between the immune function of T cells in peripheral blood with the virus replication and hepatitis C virus（HCV）-cAg expression in the patients with chronic hepatitis C（CHC）. Methods Peripheral blood T lymphocytes subpopulation, HCV-RNA and HCV core antigen （HCV-cAg） in 63 healthy people undergoing the physical exami- nation （control group） and 85 cases of CHC（CHC group） were analyzed by the flow cytometry,real-time PCR and ELISA,respec- tively. Results The percentages of total T cells,T4 cells, T8 cells, double negative T cells （DNT） and double positive T cells （DPT） in the CHC group were （67.37±10.43）%,（37. ll±10.28）%,（21.63±8.87） %,（7.80±4.57） % and （0.20±0.29）%, respectively,the absolute contents were in turn （0.70±0.44）×10^9/L, （0.37 ± 0.22） × 10^9/L, （0. 22±0. 17） × 10^9/L, （0.08± 0.06） × 10^9/L and （0.19 ± 0.68） × 10^7/L, respectively. The ratio of T4/T8 was （2.18 ± 1.26）%. The results indicated that the percentage of T8 cells in the CHC patients was decreased obviously（P〈0.01）, which resulted in the ratio of T4/T8 raising（P〈0.05） ; meanwhile, the absolute contents of the total T cells, T4 cells, T8 cells and DNT were all decreased obviously （P〈 0.05 ） moreover,the percentage of T4 cells and DNT in the patients with HCV-RNA positive and HCV-cAg positive was significantly in- creased （P〈0.05）. Conclusion When HCV replicating in the patients with CHC,the T lymphocyte subpopulation has obvious ab- normity. The low immune function or immune tolerance of T cells may be the important cause of recurrence and uncurability of CHC.

关 键 词：丙型肝炎 T细胞亚群 流式细胞术 聚合酶链反应 

分 类 号：R512.63[医药卫生—内科学]