痰瘀阻络模型大鼠血液流变学及红细胞膜生物学研究  

Biological Study of Hemorheology and Erythrocyte Membrane of Model Rats with Stagnation of Phlegm and Blood

作　　者：凌爽[1] 毕悦[1] 翟慧颖[1] 杨茂波 牛雯颖[1] 纪博硕 肖洪彬[1] 

机构地区：[1]黑龙江中医药大学,黑龙江哈尔滨150040

出　　处：《中医药学报》2015年第2期40-43,共4页Acta Chinese Medicine and Pharmacology

基　　金：国家自然科学基金项目(81173186)

摘　　要：目的:探讨痰瘀阻络模型大鼠的血液流变学和红细胞膜的生物学特征变化。方法:选用SD雄性大鼠40只,按体质量随机分为四组,每组10只。10天组分为空白组和痰瘀阻络模型组两组;20天组同样分为空白组和模型组两组。模型组分别采用脂乳剂连续灌胃10天和20天复制高脂血症模型,取血测定血液流变学和红细胞膜组分相关指标。结果:与空白组比较,痰瘀阻络模型组大鼠血浆总胆固醇(CHO)﹑甘油三酯(TG)﹑低密度脂蛋白胆固醇(LDL-C)﹑血糖(GLU)含量均显著升高(P<0.05),高密度脂蛋白胆固醇(HDL-C)含量显著降低(P<0.05);全血黏度﹑血浆黏度均显著升高(P<0.05,P<0.01);Na+-K+-ATP酶活力﹑SOD活力均不同程度降低(P<0.05,P<0.05),MDA含量显著升高(P<0.05),唾液酸含量有降低趋势,但无显著性差异;膜胆固醇含量极显著升高(P<0.01)。结论:痰瘀阻络模型中胆固醇含量的升高,引起血浆黏度的增大,可能导致红细胞膜上SOD酶活力、Na+-K+-ATP酶活力降低,MDA含量升高,膜胆固醇含量显著升高,同时唾液酸含量也有降低趋势。Objective：To investigate the changes of biological characteristics of hemorheology and erythrocyte membrane of model rats with stagnation of phlegm and blood.Methods：40 SD male rats were randomly divided into four groups, with ten in each group.Ten-days group was subdivided into a blank group and a model group;twenty-days group was also subdivided into a blank group and a model group.High fat emulsion was continuously given to model rats intragas-trically for 10 days and 20 days respectively, and then determine the hemorheology indexes and activity of erythrocyte membrane.Results：Compared with the blank group, the serous CHO, TG, LDL-C and GLU were increased signifi-cantly （P〈0.05）, and HDL-C was decreased obviously （P〈0.05）; the whole blood viscosity and the plasma vis-cosity were increased obviously （ P〈0 .05 , P〈0 .01 ）;Na ＋-K＋-ATP enzyme and SOD activity were decreased ob-viously（P〈0.05, P〈0.05）, and the contents of MDA and membrane cholesterol were increased significantly （P〈0.05）, and SA had a decreased tendency.Conclusion：Models with stagnation of phlegm and blood can cause the in-crease of cholesterol, and the plasma viscosity is also increased.It might lead to the decrease of SOD and Na ＋-K＋-ATP enzyme, meanwhile, MDA and membrane cholesterol contents have been changed to different degrees.Meanwhile, the content of SA has a decreased tendency.

关 键 词：痰瘀阻络模型 血液流变学 红细胞膜 生物学特征 

分 类 号：R285.5[医药卫生—中药学]