基于原子力显微镜的姜黄素对肺癌细胞机械特性的影响研究  

Effect of Curcumin on Mechanical Properties of Lung Cancer Cells Based on the Atomic Force Microscope

作　　者：王晓慧[1] 栗亚[1] 蔡文辉[1] 刘宏[1] 孙力[1] 姜颖[1] 史海蛟[1] 

机构地区：[1]黑龙江中医药大学,黑龙江哈尔滨150040

出　　处：《中医药学报》2015年第2期46-48,共3页Acta Chinese Medicine and Pharmacology

基　　金：黑龙江省教育厅科学技术研究项目(11551462)

摘　　要：目的:基于Hertz弹性接触模型,基于原子力显微镜(AFM)研究姜黄素对肺癌细胞的机械特性尤其是弹性模量的影响。方法:利用核小体酶联免疫吸附试验检测姜黄素作用后的细胞凋亡,利用AFM检测癌细胞的表面形貌和机械特性。结果:姜黄素明显诱导癌细胞产生凋亡,凋亡细胞的弹性模量和硬度明显增加。结论:姜黄素诱导细胞凋亡的机制提示细胞骨架的参与,癌细胞的硬度增加可能进一步抑制细胞的远处迁移和运动。Objective：To study the effect of curcumin on mechanical properties of lung cancer cells, especially on the modulus of elasticity.Methods：Use nucleosome enzyme-linked immunosorbent assay to detect cell apoptosis after curcu-min role,and use atomic force microscope to detect the surface morphology and mechanical properties of cancer cells.Re-sults：Curcumin significantly induced apoptosis of cancer cells, and the elastic modulus and hardness of the apoptosis cells increased significantly.Conclusion：The mechanism of apoptosis induced by curcumin prompts the participation of cytoskeleton,and the increased hardness of cancer cells can further inhibit cell migration and movement in the distance.

关 键 词：姜黄素 原子力显微镜 肺癌 机械特性 凋亡 

分 类 号：R284.2[医药卫生—中药学]