209例HIV-1阳性静脉吸毒者中HCV基因型研究  

Characterization of HCV genotypes among 209 HIV-1 positive intravenous drug users in Guangdong

作　　者：况轶群[1] 黄旭和[1] 颜瑾[2] 周平平[1] 于国龙[2] 鄢心革[2] 刁丽梅[2] 何翔[1] 

机构地区：[1]广东省疾病预防控制中心广东省公共卫生研究院,广东广州511430 [2]广东省疾病预防控制中心

出　　处：《华南预防医学》2015年第2期124-131,共8页South China Journal of Preventive Medicine

基　　金：广东省医学科研基金(No.B2012073);国家自然科学基金(No.31200130;No.81371812);广东省自然科学基金(No.S2013010011860);广东省科技计划项目(No.2012B031800267)

摘　　要：目的了解2007—2012年广东省内HIV-1感染的静脉吸毒者（IDUs）中HCV基因型的种类和亚型分布情况。方法对2007—2012年HIV-1抗体阳性IDUs样本进行HCV抗体检测,对HCV抗体阳性样本提取RNA,用反转录巢式聚合酶链反应方法扩增HCV核心基因Core片段和非结构基因NS5B片段,目的片段测序后通过分子系统发育树进行基因型分析。结果 209份样本分别来自惠州、东莞、阳江、云浮、清远、佛山、中山和江门,以阳江和东莞为主,占36.84%（77/209）,以男性为主,占93.78%（196/209）,年龄在18-49岁之间。HCV抗体阳性166份,阳性率为79.4%。进化树显示样本序列分别与标准株1a、1b、2a、3a、3b和6a等6种亚型聚集在一起,la、lb、2a、3a、3b、6a基因亚型构成比分别为10.6%、9.9%、1.4%、22.7%、11.3%、44.0%。结论 6a是广东省内被检测HIV-1感染IDUs中HCV主要流行亚型,其次为3a亚型。Objective To understand the genotypic distribution of HCV among HIV-1 /HCV co-infected injection drug users（ IDUs）. Methods Plasma samples of HIV-1 seropositive IDUs identified from2007 to 2012 were collected for the HCV sero-test. RNA genome of HCV-positive samples was purified,and then the HCV Core and NS5 B fragments were amplified by RT-nested PCR and sequenced. The target gene sequences were used for HCV genotyping / subtyping via phylogenetic analyses. Results A total of209 samples of IDUs aged between 18 and 49 years were collected from cities of Huizhou,Dongguan,Yangjiang,Yunfu,Qingyuan,Foshan,Zhongshan,and Jiangmen and 196 cases were males（ 93. 78%）.The samples of the IDUs were mainly from Yangjiang and Dongguan（ 36. 84%）. Among the 209 samples tested,166（ 79. 4%） were found to be positive for HCV antibody. Phylogenetic trees showed that HCV sequences were clustered with standard strains la（ 10. 6%）,lb（ 9. 9%）,2a（ 1. 4%）,3a（ 22. 7%）,3b（ 11. 3%）,and 6a（ 44. 0%）,respectively. Conclusion The most predominant subtype was HCV 6a in IDUs infected with HIV-1 in Guangdong Province,followed by the 3a subtype.

关 键 词：HIV感染 肝炎 丙型 基因型 

分 类 号：R512.63[医药卫生—内科学]