米力农治疗心力衰竭大鼠的钙调节机制研究  

Calcium Regulating Mechanism Research of Milrinone on Heart Failure Rat

作　　者：邱红[1] 朱红枫[2] 张若松[1] 

机构地区：[1]宁波市妇女儿童医院,浙江宁波315000 [2]泸州医学院附属医院,四川泸州646000

出　　处：《儿科药学杂志》2015年第5期4-6,共3页Journal of Pediatric Pharmacy

摘　　要：目的:通过观察米力农对心力衰竭大鼠心肌钙离子(Ca2+)、基质金属蛋白酶-2(MMP-2)、肌钙蛋白酶Ⅰ(c TnⅠ)、肌酸激酶(CK)、肌酸激酶同工酶(CK-MB)的影响,探讨米力农通过细胞内钙调节治疗心力衰竭的机制。方法:将30只健康雄性SD大鼠随机分为对照组、心力衰竭组、米力农组各10只,对照组腹腔注射生理盐水0.75 m L/kg,每周2次,共7周;心力衰竭组、米力农组建立心力衰竭模型:盐酸阿霉素用生理盐水配成2 mg/m L的注射液,以0.75 m L/kg(1.5 mg/kg)腹腔注射,每周2次,共7周;米力农组在第7周给予米力农50μg/kg,加注射用水配制成0.5 m L的注射液经腹腔注射,每天1次,共7 d。分别在末次给药后24 h检测三组大鼠心肌Ca2+、血清MMP-2、c TnⅠ及心肌酶CPK、CK-MB水平。结果:心力衰竭组心肌Ca2+及血清MMP-2、c TnⅠ、CPK、CK-MB水平均比对照组(P均<0.01)和米力农组高(P<0.05)。结论:心力衰竭时心肌肌浆网钙泵活性受损,米力农可有效促进Ca2+内流和肌浆网钙的释放产生正性肌力作用,从而改善心脏功能,减轻心肌受损。Objective： To research the changes of myocardial calcium ions （Ca2＋）, matrix metalloproteinase-2 （MMP-2） and troponin-I （cTnI）, creatiue kinase （CK）, creatine kinase isoenzyme （CK-MB） of heart failure rat with milrinone, and investigate the mechanism of milrinone in treating heart failure by regulating intracellular calcium. Methods： Thirty rats were divided into control group, heart failure group, milrinone group. The control group was given saline solution 0.75 mL/kg by intraperitoneal injection, 2 times a week, a total of 7 weeks. Rats of heart failure group and milrinone group were builded model of heart failure. Hydrochloric acid doxorubicin mixed with physiological saline injection into 2 mg/mL, the rats of heart failure model were given 0.75 mL/kg （ 1.5 mg/kg） by intraperitoneal injection, 2 times a week, a total of 7 weeks; Milrinone （50μg/kg） plus injection water to mixture injection, milrinone group were given 0.5 mL mixture injection by intraperitoneal injection, once a day, a total of 7 days. Detected the levels of Ca^2＋, MMP-2, cTnI and myocardial enzymes CPK, CK-MB 24 hours after the last administration. Results： Compared with the control group, the levels of Ca2＋, MMP-2, cTnI, CPK, CK-MB were increased in the heart failure group （ P〈0.01 ） and milrinone group （P〈0. 05 ）. Conclusion： Activity of myocardial sarcoplasmic reticulum calcium pump is impaired in heart failure, Milrinone can effectively promote calcium influx and sarcoplasmic reticulum calcium release to produce a positive inotropic effect, relieve heart failure.

关 键 词：心力衰竭 心肌酶 基质金属蛋白酶 肌钙蛋白 钙通道 

分 类 号：R541.6[医药卫生—心血管疾病]