ILLLI对银屑病患者外周血CD4+T细胞Fas蛋白表达的影响  

The Study on the Expression of Fas Protein of CD4+ T Cell in the cases of psoriasis by low level He-Ne laser irradiation

作　　者：聂凡[1,2] 张慧国[1,2] 袁霞雯[1,2] 朱菁[1,2] 施虹敏[1,2] 张美珏[1,2] 包晓青[1,2] 周琳[1,2] 秦梅香[1,2] 

机构地区：[1]上海第二医科大学附属仁济医院 [2]上海市激光医学研究中心,上海200001

出　　处：《应用激光》2002年第3期345-348,共4页Applied Laser

摘　　要：目的:探索ILLLI治疗对银屑病患者外周血CD4+T细胞Fas蛋白表达的影响。方法:取患者及正常对照组静脉血10ml,肝素抗凝,等份分别加入4支试管中,1支为未照射组,3支照射组分为30、60和120分钟,以输出功率5mW的氦氖激光照射,并进行荧光染色和流式细胞分析。结果:照射前银屑病组表达Fas蛋白的CD4+T细胞百分比为21.4±3.1％,较正常对照组的16.8±2.1％显著增加,差异有显著性,p<0.05。正常对照组在激光照射30分钟、60分钟和120分钟,表达Fas蛋白的CD4+T细胞百分比分别为18.0±5.3％、16.4±5.2％和19.8±6.7％,与照射前差异无显著性,p>0.05;而患者组在激光照射30分钟、60分钟和120分钟时,表达Fas蛋白的CD4+T细胞百分比分别为24.3±2.8％、28.3±5.3％和30.5±7.9％,可以看出,随着照射时间的延长,表达Fas蛋白的CD4+T细胞的数量不断增加,60分钟和120分钟,表达Fas蛋白的CD4+T细胞百分比较照射前增加显著,有统计学差异,p<0.05。结论:银屑病的发病机制可能与细胞凋亡失控等多种因素有关。激光治疗银屑病的机制可能与它下调IL—8水平、上调TNF—(表达、纠正患者的凋亡失控状态有关。Objective:To investigate the influence on the Expression of Fas Protein of CD4 + T Cell in the cases of psoriasis by low level He-Ne laser irradiation. Methods: the expression of CD4 + T Fas protein was determined in the casee of psoriasis(n=5) pre and post-low level laser irradiation(30 min,60min and 120min)by flow cytometry as compared with the control(n=5). Results:In the cases of psoriasis,the expression of CD4 + T FAS protein 21. 4 ±3. 1% was increased significantly than that of control group 16. 8±2. 1% pre -irradiation, p<0. 05;in the control,there is no difference between pre and post- irradiation,p>0. 05;in the cases group, the expression of CD4 + T Fas protein wae positivelly corelated to the irradiation times, when the energy density arrived to 22. 92J/cm2(60 minutes)and 45. 84J/cm2(120minutes) , the expression of CD4 + T T Fas protein was increased significantly as compared with pre - irradiation, p<0. 05. Conclusion: The expression of CD4 + T FAS protein may be increased by low level He-Ne laser irradiation ,therefore, the uncontrolled status of apoptosis could be corrected.

关 键 词：银屑病 激光血管内照射 CD4+T细胞 FAS蛋白 

分 类 号：R758.63[医药卫生—皮肤病学与性病学]