荧光定量PCR监测抑制剂对低温SBR微生物的影响  

Quantitative PCR quantification of bacteria in low temperature SBR activated sludge influenced by chlorate

作　　者：张朝升[1] 王竞茵 荣宏伟[1] 邓杰[1] 黄小鹏 江秀贤 伍志趼 

机构地区：[1]广州大学省部共建教育部珠江三角洲水质安全与保护实验室,广东广州510006 [2]广州市净水有限公司,广东广州510655

出　　处：《广州大学学报（自然科学版）》2015年第1期69-75,共7页Journal of Guangzhou University:Natural Science Edition

基　　金：国家自然科学基金资助项目(51178125;21477027);广东省自然科学基金资助项目(S2013010013943);广东省科技计划资助项目(2012B030800005)

摘　　要：为研究氯酸盐抑制剂对SBR活性污泥中微生物的影响,采用序批式活性污泥反应器(SBR),在低温(15℃)条件下通过向反应器定时投加氯酸盐抑制剂,成功抑制系统中亚硝酸盐氧化菌(NOB)的活性,氨氮去除率和亚硝酸盐积累率均达到90%以上,实现了城市污水短程硝化的快速启动.采用实时荧光定量PCR对氨氧化菌(AOB)、亚硝酸盐氧化菌(NOB)和总细菌进行菌群定量分析,分析结果表明,氯酸盐抑制剂对NOB具有选择抑制性,对AOB的活性没有明显影响,而对NOB的活性抑制在短时间内是不可恢复的.An activated sludge sequencing batch reactor（SBR） was developed to investigate the nitrogen remov- al from municipal wastewater based on the partial nitrification. SBR added chlorate was applied to inhibit the ac- tivity of nitrite-oxidizing bacteria （NOB） in low temperature. The results showed that the NH＋4 - N removal effi- ciency and the nitrite accumulation rate of effluent were all above 90%. The quantitative PCR was used to ana- lyze the quantification of ammonia oxidizing bacteria（ AOB）, nitrite-oxidizing bacteria（NOB） and total bacteria in SBR. The results of real-time PCR revealed chlorate has its selective inhibition on NOB, without affecting the growth rate of AOB. It was also found that NOB inhibition caused by chlorate can＇t recover in a short time.

关 键 词：氯酸盐 NOB抑制 短程硝化 

分 类 号：X703[环境科学与工程—环境工程]