离子色谱-积分脉冲安培检测法测定D-核糖  

Determination of D-ribose by ion chromatography with integrated pulsed amperometric detection

作　　者：燕会丹 夏金梅[2] 许晨[2] 李军[1] 钟添华[2] 

机构地区：[1]厦门大学化学化工学院,厦门361005 [2]国家海洋局第三海洋研究所,海洋生物遗传资源重点实验室,厦门361005

出　　处：《食品科技》2015年第4期344-348,共5页Food Science and Technology

基　　金：海洋公益性专项(201105029);国家重大科学仪器专项(2013YQ170525);厦门市海洋经济发展专项资金项目(13CZP003HJ05)

摘　　要：采用离子色谱-积分脉冲安培检测法（IC-IPAD）测定D-核糖,采用Carbo PacTM PA10色谱柱进行分离,60 mmol/L Na OH溶液为淋洗液,流速0.6 m L/min,柱温30℃,ED3000安培检测器,Au工作电极,Ti对电极,Ag/Ag Cl复合参比电极。以外标法定量,D-核糖质量浓度为3.60~43.2mg/L范围内峰面积呈良好的线性关系（R2=0.9999）,检出限及定量限分别为0.200、0.620 ng,精密度实验的相对标准偏差（RSD）为0.524%,平均回收率为101%。因此,IC-IPAD可用于D-核糖含量的测定,且操作简便、无需衍生、分离效果好、灵敏度高。An analytical method was proposed and established for determination of D-ribose by ion chromatography with integrated pulsed amperometric detection （IC-IPAD）. The separation was performed on a 250 mmx4 mm CarboPacTM PAl0 column at 30 ~C by HPLC equipped with an ED3000 detector. The mobile phase was 60 mmol/L sodium hydroxide with a flow rate of 0.6 mL/min. The quantification was performed by an external standard approach. The concentration linear range for D-ribose was 3.60-43.2 mg/L（RZ=0.9999）. The relative standard deviation of precision was 0.524%. The detection and quantification limits for D-ribose were 0.200 ng and 0.620 ng, respectively. The average recovery ration for D-ribose was 101%. IC-IPAD was proved to be suitable for the determination of D-ribose with its convenience, rapidness, and sensitivity.

关 键 词：D-核糖 离子色谱-积分脉冲安培检测法 糖类检测 

分 类 号：O657.75[理学—分析化学]