响应面法优化枯草芽孢杆菌NS178产芽孢发酵工艺  

Optimization of Sporulation Fermentation Process of Bacillus subtillus NS178 by Response Surface Methodology

作　　者：王西祥[1] 丁延芹[1] 杜秉海[1,2] 姚良同[1] 祁国振 葛科[1] 刘凯[1] 

机构地区：[1]山东农业大学生命科学学院,山东泰安271018 [2]山东农业大学农业微生物学重点实验室,山东泰安271018

出　　处：《山东农业科学》2015年第4期59-65,共7页Shandong Agricultural Sciences

基　　金：农业部公益性行业科研专项经费项目(200903018)

摘　　要：应用单因素设计法对影响内生枯草芽孢杆菌NS178发酵液菌落数的因子进行筛选,得到3个主要影响因子,分别为葡萄糖、(NH4)2SO4、K2HPO4。采用Box-Behnken Design法对发酵培养基进行优化,通过响应面法分析获得最佳配方为葡萄糖2.29%、(NH4)2SO40.3%、K2HPO40.23%。对温度、初始p H值、装液量3组培养条件进行优化,最佳条件为33°C、p H 7.6、50 m L。在最佳配方和培养条件下,NS178发酵液菌落数为34.50×108cfu/m L;培养72 h时芽孢率达到75.8%;优化后比优化前发酵液抑菌圈直径增加67%。Three factors mainly influencing the colony number of endophytic Bacillus subtillus NS178 in fermentation liquid were screened out by single-factor experiments, which were glucose, （ NH4 ） 2 SO4 and K2 HPO4 .The Box-Behnken Design was applied to optimize the fermentation medium for NS178, and the op-timum one was obtained as 2.29%glucose, 0.3%（NH4）2SO4 and 0.23%K2HPO4 after the analysis of re-sponse surface methodology.The culture conditions were also optimized, and the optimal conditions were tem-perature as 33℃, pH value as 7.6 and fluid volume as 50 mL.Under the optimal fermentation conditions, the yield of NS178 was 34.50×10^8 cfu/mL;the spore rate could reach 75.8%after cultured for 72 hours;and the diameter of inhibition zone increased by 67%compared to that before fermentation.

关 键 词：枯草芽孢杆菌 芽孢 响应面 抑菌圈 

分 类 号：S144[农业科学—肥料学]