补体C3在大鼠肝脏缺血再灌注中的作用  

The role of the complement C3 in rat's hepatic ischemia reperfusion

作　　者：何志恩 饶艳[2] 邓少剑[1] 柯剑娟[3] 

机构地区：[1]广州市番禺区中医院麻醉科,511400 [2]中山大学中山眼科中心麻醉科,广州510060 [3]武汉大学中南医院麻醉科,430071

出　　处：《中华临床医师杂志（电子版）》2015年第8期89-92,共4页Chinese Journal of Clinicians(Electronic Edition)

基　　金：番禺区科技计划项目(2012-Z-03-01)

摘　　要：目的通过观察补体C3在大鼠肝脏缺血再灌注模型中的变化,探讨补体C3在大鼠肝脏缺血再灌注中的作用。方法健康雄性SD大鼠60只,180~200 g;随机分成假手术组（S组）,全肝缺血再灌注1 h组（I/R 1 h组）,全肝缺血再灌注3 h组（I/R 3 h组）,全肝缺血再灌注6 h组（I/R 6 h组）和全肝缺血再灌注24 h组（I/R 24 h组）,共5组,每组12只。建立全肝缺血再灌注模型,于再灌注时1、3、6、24 h选取肝脏标本行HE染色,在显微镜下观察肝脏的病理学改变,荧光实时定量PCR法检测补体C3 m RNA表达,Western blot法检测补体C3的表达。结果 （1）S组可以观察到肝脏组织的结构基本正常,I/R（1 h,3 h,6 h,24 h）4组均有不同程度肝组织的损伤,并且损伤程度随着缺血再灌注的时间延长而逐渐加重。（2）与S组比较,I/R 1 h组的补体C3 m RNA的表达差异无统计学意义（P〉0.05）;随着时间延长,补体C3 m RNA的表达逐渐升高,于24 h时达到最高峰,与其他4组比较具有明显的统计学意义（P〈0.05）;补体C3的蛋白表达也随着时间的延长逐渐升高,I/R 1 h组的蛋白表达与S组比较无明显上调（P〉0.05）,补体C3蛋白含量在I/R 24 h组中表达达到高峰,与其他I/R组相差异有统计学意义（P〈0.05）。结论补体C3的活化参与了大鼠肝脏缺血再灌注损伤。Objective To research and analyze the role of the complement C3 by observing the change of complement C3 in the model of hepatic ischemia reperfusion. Methods Sixty healthy and male SD rats, each weighing180-200 g, were randomly assigned to sham operation group （group S）, hepatic I/R 1 hour （I/R 1 h group）, hepatic I/R 3 hours （I/R 3 h group）, hepatic I/R 6 hours （I/R 6 h group）, hepatic I/R 24 hours （I/R 24 h group）. There were 5 groups, 12 rats in each group, which built the model of hepatic ischemia reperfusion. Hepatic specimens were obtained at 1, 3, 6, and 24 h of reperfusion and stained with haematoxylin and eosin for examination of histological changes of the liver, and for expression of complement C3 mRNA by using real-time PCR and for expression of complement C3 by using Western blot. Results The structure of liver tissue was basically normal from the observation of group S, while the structures of liver tissue from I/R（1 h, 3 h, 6 h, 24 h） were injured in varying degrees and the degrees of injury were aggravated with the prolonging time of reperfusion. Compared with group S, the expression of complement C3 mRNA had no statistics meanings （P〉0.05）;with the prolonging of time, the expression of complement C3 mRNA was higher and higher and reached the peak at 24 hours, which had the obvious statistics meanings compared with the other four groups （P〈0.05）; the expression of complement C3’protein was higher and higher with the prolonging of time, and compared with group S, the expression of protein from I/R 24 hour was not clearly increased （P〉0.05）. the content of the expression of complement C3 protein reached the peak in the group I/R 24 h, and compared with other I/R group, it had the significant difference and statistics meanings （P〈0.05）. Conclusion Activation of complement C3 is involved in hepatic I/R injury rats.

关 键 词：肝 再灌注损伤 补体C3 

分 类 号：R657.3[医药卫生—外科学]