姜黄素眼用脂质体的角膜透过性研究  

The Effect of N- trimethyl Chitosan as Coating Materials on Corneal Permeability of Curcumin

作　　者：张婧[1] 李翔[2] 罗云[1] 梁新丽[1] 廖正根[1] 

机构地区：[1]江西中医药大学现代中药制剂教育部重点实验室,南昌330004 [2]江西中医药大学中药固体制剂制造技术国家工程研究中心,南昌330006

出　　处：《江西中医药大学学报》2015年第2期79-81,共3页Journal of Jiangxi University of Chinese Medicine

基　　金：国家自然科学基金项目(81202927);江西省教育厅青年基金项目(GJJ12535;GJJ12536);江西省卫生厅中医药科研计划项目(2012A160;2012A157);江西省青年科学基金项目(20122BAB215018;20132BAB215022;20114BAB215038);江西省科技支撑计划项目(20123BBG70181)

摘　　要：目的:研究姜黄素眼用脂质体的角膜透过性。方法:采用乙醇注入法制备姜黄素脂质体,以三甲基壳聚糖对脂质体囊泡进行包覆;采用过膜法、粒径及电位测定仪对脂质体进行理化性质分析;采用离体兔角膜透过实验及共聚焦激光扫描显微镜成像技术,对三甲基壳聚糖包覆的脂质体的角膜透过作用进行研究。结果:三甲基壳聚糖对脂质体的包覆对脂质体的包封率无明显影响,但粒径、电位有所增大,同时角膜透过显著高于未包覆脂质体组,随着作用时间的延长,可促进脂质体囊泡渗透至角膜上皮深层60μm处,并主要以细胞旁路途径发生角膜透过。结论:该研究表明三甲基壳聚糖对脂质体囊泡具有明显的角膜促透作用。Objective：To study the effect of N-trimethyl chitosan as coating materials on corneal permeability of curcumin.Methods：Curcumin-loaded liposomes were prepared by ethanol injection method, and the vesicles were coated with trimethyl chitosan.Mem-brane filter, dynamic light scattering and electrophoretic light scattering were employed to study the physicochemical parameters.Cor-neal permeability of trimethyl chitosan-coated liposomes was carried out by transcorneal permeation study and confocal laser scanning microscopy （ CLSM） .Results：It was shown that the entrapment efficiency was not changed significantly with enlarged size and zeta po-tential with the coating of TMC.The TMC-coated liposomes produced the more pronounced corneal permeation, compared with the uncoated preparations.With the increased contact time, the fluorescence in rabbit corneal epithelium can be detected down to 60 μm below the surface via paracellular route.Conclusion：TMC has significant effect on the transport of curcumin through rabbit cornea in vitro.

关 键 词：三甲基壳聚糖 姜黄素 角膜透过 共聚焦激光扫描显微镜成像 

分 类 号：R285.5[医药卫生—中药学]