荧光定量PCR检测病毒性心肌炎患者血浆循环DNA及其临床意义  

Real-time fluorescent quantitative PCR in detection of plasma circulating DNA of patients with viral myocarditis and its clinical significance

作　　者：杨大鹏[1] 

机构地区：[1]北华大学附属医院检验科,吉林吉林132011

出　　处：《中国民康医学》2015年第9期11-13,共3页Medical Journal of Chinese People’s Health

基　　金：吉林省教育厅"十二五"计划科学技术研究项目(项目编号:吉教科合字【2011】第135号

摘　　要：目的:利用实时荧光定量PCR技术来检测病毒性心肌炎患者血浆循环DNA水平。方法:分别收集健康人和病毒性心肌炎患者的血浆样本,用实时荧光定量PCR方法检测样本中的血浆循环DNA。结果:病毒性心肌炎患者的血浆循环DNA的CT值为25.22±1.26,明显低于健康人30.66±1.52,有显著性差异(P<0.01)。结论:实时荧光定量PCR技术检测血浆循环DNA可以成为诊断病毒性心肌炎的一种方法。Objective: To detect plasma circulating DNA levels of patients with viral myocarditis with real-time fluorescent quantitative PCR. Methods:Plasma samples from healthy subjects and the patients with viral myocarditis were collected, and the lev-els of plasma circulating DNA were evaluated by real-time fluorescent quantitative PCR. Results:The CT value of the circulating DNA in plasma of the patients with viral myocarditis (25. 22±1. 26) was significantly lower than that of the healthy subjects (30. 66±1. 52) (P<0. 01). Conclusions: Real-time fluorescent quantitative PCR technique for the detection of plasma circulating DNA can be a method for diagnosis of viral myocarditis.

关 键 词：实时荧光定量PCR 病毒性心肌炎 血浆循环DNA 

分 类 号：R542.21[医药卫生—心血管疾病]