甘肃省张掖地区苜蓿花叶病病原的检测  

Detection of pathogenic organisms in Medicago sativain Zhangye,Gansu Province

作　　者：文朝慧[1,2] 南志标[1] 

机构地区：[1]兰州大学草地农业科技学院、草地农业生态系统国家重点实验室,甘肃兰州730020 [2]甘肃出入境检验检疫局,甘肃兰州730010

出　　处：《草业学报》2015年第4期121-126,共6页Acta Prataculturae Sinica

基　　金：公益性行业(农业)科技专项(No.201303057);国家质检总局科技计划项目(2012IK270);甘肃出入境检验检疫局科技计划项目(2011GK001)资助

摘　　要：2011年7月在甘肃省张掖地区发现发生花叶病的苜蓿田块,地块中病株呈现黄斑花叶、叶柄扭曲及整株矮化的症状。为明确其病原,采集病株后利用血清学和分子生物学方法对病样进行了检测。DAS-ELISA、RT-PCR-RFLP及病毒CP基因序列测定分析结果表明,苜蓿病样受到番茄花叶病毒(Tomato mosaic virus,ToMV)和苜蓿花叶病毒(Alfalfa mosaic virus,AMV)的复合侵染。这是国际上首次报道番茄花叶病毒对苜蓿的侵染,讨论了由这两种病毒引致病害的发生与防治。Two Medicago sativa plants with severe leaf mosaic symptoms were collected from alfalfa fields in Linze County,Gansu Province,in July 2011.The two samples were tested by enzyme-linked-immunosorbent assay （ELISA）and reverse transcription polymerase chain reaction （RT-PCR）.The tested specimens were negative for Cucumber mosaic virus,Tobacco streak virus,Watermelon mosaic virus,Potato virus X,Potato virus Y,Tobacco ringspot virus,Tomato ringspot virus and Bean yellow mosaic virus.However,Tomato mo-saic virus （ToMV）and Alfalfa mosaic virus （AMV）were detected.The 687 and 351 bp fragments obtained from PCR with appropriate species-specific ToMV and AMV primers showed 99% nucleotide similarity with published ToMV CP and AMV CP gene sequences.A 805 bp PCR products was obtained using genus-specific Tobamovirus primers and the presence of ToMV was confirmed by RT -PCR -RFLP.To the best of our knowledge,this is the first report of ToMV in alfalfa.

关 键 词：苜蓿 番茄花叶病毒 苜蓿花叶病毒 病毒病 

分 类 号：S435.4[农业科学—农业昆虫与害虫防治]