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作 者:李华雄[1,2] 程明军 苏月贵[1,3] 李志龙[1,4] 蒋维明 张红芬 郑名敏[1] 荣廷昭[1] 周树峰[1] 吴元奇[1] 曹墨菊[1] 唐祈林[1]
机构地区:[1]四川农业大学玉米研究所,四川温江611130 [2]内江市农业科学院,四川内江641000 [3]广西农业科学院,广西南宁530007 [4]宜宾市农业科学院,四川宜宾644000 [5]石林县长湖镇农业综合服务中心,云南昆明652203
出 处:《草业学报》2015年第4期157-163,共7页Acta Prataculturae Sinica
基 金:973项目(2014CB13870);国家自然科学基金项目(31371640);四川省饲草育种攻关项目资助
摘 要:多色基因组原位杂交(multi-color genomic in situ hybridization,McGISH)鉴定结果表明,MTPP-58有58条染色体,其中11条染色体来自玉米,28条染色体来自四倍体多年生大刍草,17条染色体来自指状摩擦禾和2条玉米与四倍体多年生大刍草易位的染色体。2012-2013年对MTPP-58的饲草产量、品质、越冬率以及繁殖特性研究表明,第1年鲜、干草产量分别为93.845t/hm2和22.083t/hm2,第2年鲜、干草产量分别为82.851t/hm2和14.394t/hm2;粗蛋白(CP)为10.48%,干茎叶比为0.71,越冬率为100%。MTPP-58下部茎干扦插成活率为51.69%,分株成活率为95.50%。多种属聚合远缘杂交创制的MTPP-58聚合了玉米、大刍草、摩擦禾的优良特性,植株根系发达,生长繁茂,分蘖和抗寒性强,生产性能优,可无性繁殖,是一种多种属间杂交创制的新型多年生饲草。Multi-color genomic in situ hybridization (McGISH)was employed to identify the chromosomal com-position of Yuqilin 58 (MTPP-58),derived from the tri-generic hybridization of Zea mays ,Zea perennis and Tripsacum dactyloides .GISH (Genomic in situ hybridization)showed that MTPP-58 is an aneuploid with 58 chromosomes;11,28 and 17 chromosomes came from Z .mays ,Z .perennis and T.dactyloides respectively. Translocation of chromosomes between Z .mays and Z .perennis accounted for the balance.Assessment of forage yield,forage quality,over wintering rate and reproductive characteristics showed that the fresh and dry hay yield of MTPP-58 were 93.845 t/ha and 22.083 t/ha respectively,in the first year,and 82.851 t/ha and 14.394 t/ha respectively,in the second year.The crude protein content of the forage was 10.48% with a leaf/stem ratio of 0.71.Winter survival rate was 100%.MTPP-58 displayed a number of desirable characteristics including good growth,a well-developed root system,good tillering ability and good reproductive potential suggesting it has good potential for ongoing development.
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