点带石斑鱼血液指标、血细胞分类与发生的观察  

Hematological Indices,and Classification and Hematopoiesis of Blood Cells in Malabar Grouper Epinephlus malabaricus

作　　者：毛海涛[1] 孙敬锋[1] 王庆奎[1] 孙学亮[1] 陈成勋[1] 

机构地区：[1]天津农学院水产学院,天津市水产生态及养殖重点实验室,天津300384

出　　处：《水产科学》2015年第4期214-219,共6页Fisheries Science

基　　金：国家自然科学基金资助项目(31270456);国家“十二五”科技支撑项目(2011BAD13B00);天津市科技支撑计划项目(06YFGZNC08100);天津市高等学校创新团队基金资助项目(TD12-5018)

摘　　要：对20尾点带石斑鱼进行了血液指标、血细胞分类和发生的研究。血液指标经测定:红细胞密度为(1.89±0.48)×106个/mm3,白细胞密度为(1.65±0.34)×104个/mm3;血红蛋白含量为(7.11±0.67)g/L;红细胞的渗透脆性为(0.54±0.02)%,红细胞比容为(36.7±0.67)%,红细胞沉降率为(2.50±0.25)mm/h。利用Wright’s-Giemsa混染法对点带石斑鱼外周血液以及肝脏、脾脏、头肾和体肾四种造血组织染色观察发现,在外周血涂片上可区分出红细胞(包括未成熟的红细胞)、血栓细胞、淋巴细胞、嗜中性粒细胞和单核细胞,未发现嗜酸性粒细胞和嗜碱性粒细胞。其中大淋巴细胞、小淋巴细胞、嗜中性粒细胞和单核细胞在白细胞中所占的比例分别为(23.13±6.99)%、(48.97±7.42)%、(21.03±4.42)%和(6.87±2.27)%。点带石斑鱼的头肾、体肾和脾脏可以产生各种类型的血细胞,是主要的造血器官;肝脏未观察到原红细胞。The hematological indices ,and classification and development of blood cells were investigated in 20 samples of malabar grouper Epinephlus malabaricus .The numbers of erythrocyte and leukocyte were found to be （1 .89 ± 0 .48） × 106 ind/mm3 and （1 .65 ± 0 .34） × 104 ind/mm3 respectively ,with hemoglobin content of （7 .11 ± 0 .67） g/L ,and erythrocyte osmotic fragility of （0 .54 ± 0 .02）% .The hematocrit was （3 .67 ± 0 .67）% ,and the erythrocytes had sedimentation rate of （2 .50 ± 0 .25） mm/h .The observation of the stained smears of peripheral blood ,head kidney ,kidney ,liver and spleen revealed that erythrocytes （including immature erythrocyte ） ,thrombocytes ,lymphocytes ,neutrophils and monocytes were recog‐nized ,and that no ensinophilic granulocytes and basophilic granulocytes were found ,in which the large lymphocytes accounted for （23 .13 ± 6 .99）% ,small lymphocytes （48 .97 ± 7 .42）% ,neutrophils （21 .03 ± 4 .42）% and monocytes （6 .87 ± 2 .27）% .The various developmental blood cells were observed in liver , spleen ,pronephros and kidney ,indicating that the spleen ,pronephros and kidney were the developmental centre of all kinds of blood cells ,and that the primitive erythrocytes were not observed in liver .

关 键 词：点带石斑鱼 血液指标 血细胞 

分 类 号：S917[农业科学—水产科学]