镉、汞单独及联合胁迫对中华绒螯蟹的急性毒性  

Single and Joint Acute Toxicity of Cd^(2+) and Hg^(2+) to Chinese Mitten Handed Crab Eriocheir sinensis

作　　者：彭雪[1] 黄晓亮[1] 陈思涵[1] 陈上静 应雪萍[1] 

机构地区：[1]温州大学生命与环境科学学院,浙江温州325027

出　　处：《水产科学》2015年第4期220-226,共7页Fisheries Science

基　　金：国家大学生创新训练计划项目(No.201410351012);浙江省自然科学基金资助项目(No.LY13C04003);温州市科技创新团队项目(No.C20120007-07)

摘　　要：采用静水体外暴露法,根据Cd2+和Hg2+的24h全致死和无死亡质量浓度区间,按等对数间距设立6个质量浓度组,即Cd2+为25.00、52.23、109.13、228.00、476.37、993.27mg/L,Hg2+为3.00、7.26、17.58、42.57、103.06、249.53mg/L,研究了Cd2+和Hg2+对体质量48.58~87.09g的中华绒螯蟹的急性染毒;以Cd2+、Hg2+的96h半致死质量浓度为一个毒性单位,按照毒性比1∶1设置5个质量浓度组研究了镉-汞联合毒性。4d的试验结果表明,Cd2+对中华绒螯蟹的24、48、72、96h的半致死质量浓度分别为654.226、192.429、70.767、40.279mg/L;Hg2+对中华绒螯蟹的24、48、72、96h的半致死质量浓度分别为164.157、19.214、6.738、3.641 mg/L,Hg2+对中华绒螯蟹的毒性比Cd2+明显。Cd2+、Hg2+对中华绒螯蟹的安全质量浓度分别为4.994mg/L和0.079mg/L。Hg2+和Cd2+联合作用对中华绒螯蟹24、48、72、96h的相加指数分别为4.291、4.587、3.367和2.003,具有显著的协同效应。Chinese mitten handed crab Eriocheir sinensis with body weight of 48 .58—87 .09 g was exposed to Cd2＋ concentrations of 25 .00 ,52 .23 ,109 .13 ,228 .00 ,476 .37 and 993 .27 mg/L and Hg2＋ concentra‐tions of 3 .00 ,7 .26 ,17 .58 ,42 .57 ,103 .06 and 249 .53 mg/L ,respectively ,and jointly by mixing equitoxic ratio of 96 h‐median lethal concentrations （LC50 ） of Cd2＋and Hg2＋ for 4 days to understand the acute tox‐icity effect of Cd2＋ and Hg2＋ on survival of the crab .The results showed that the LC50 of Cd2＋ were 654 .226 mg/L in 24 h ,192 .429 mg/L in 48 h ,70 .767 mg/L in 72 h ,and 40 .279 mg/L in 96 h ,and the LC50 of Hg2＋ were 164 .157 mg/L in 24 h ,19 .214 mg/L in 48 h ,6 .738 mg/L in 72 h and 3 .641 mg/L in 96 h .The safety concentrations （SC） were shown to be 4 .994 mg/L for Cd2＋and 0 .079 mg/L for Hg2＋ , higher toxicity in Hg2＋ than in Cd2＋ .The joint toxicity test revealed that the additive index （AI） was 4 .291 in 24 h ,4 .587 in 48 h ,3 .367 in 72 h and 2 .003 in 96 h for equitoxic mixtures of Hg2＋ and Cd2＋ , showing the synergistic joint toxic effects of Cd2＋ and Hg2＋ .

关 键 词：Cd2+ Hg2+ 急性毒性 联合毒性 中华绒螯蟹 

分 类 号：S968.25[农业科学—水产养殖]