橡胶草根部离体培养诱导植株的研究  

Root Callus Induction of Taraxacum kok-saghyz Rodin

作　　者：贾瑞[1,2] 吴坤鑫[2,3] 王颖[2,3] 贾贤[1,2] 陈雄庭[2,3] 

机构地区：[1]海南大学农学院,海南海口570228 [2]中国热带农业科学院热带生物技术研究所农业部热带作物生物学与遗传资源利用重点实验室,海南海口571101 [3]中国热带农业科学院橡胶研究所,海南儋州571737

出　　处：《热带作物学报》2015年第4期692-697,共6页Chinese Journal of Tropical Crops

基　　金：国家自然科学基金(No.31070608);中央级公益性科研院所基本科研业务费专项资金(No.ITBBYB082;ZDZX2013023-1)

摘　　要：以橡胶草的根部作为外植体进行组织培养和快速繁殖研究。结果表明:橡胶草根部外植体防止褐化的最佳方法为MS+500 mg/L AC预培养3 d;根部愈伤组织诱导的最佳培养基为MS+0.6 mg/L 6-BA+0.3 mg/L 2,4-D,平均诱导率为90%;分化培养基为MS+0.8 mg/L 6-BA+0.3 mg/L NAA;生根培养基为1/2MS+0.2 mg/L NAA。Tissue culture and rapid propagation of Taraxacum kok-saghyz Rodin were conducted with root as the explant. The results showed that 500mg/L of activate carbon in the MS medium of pre-culture tested three days could significantly inhibit the browning of explants. The best culture medium（MS＋0.6 mg/L 6-BA＋0.3 mg/L 2,4-D） was suitable for callus induction of root, and the average induction rate was 90.0%. The differentiation medium was MS＋0.8 mg/L 6-BA ＋0.3 mg/L NAA. The rooting medium was 1/2MS＋0.2 mg/L NAA.

关 键 词：橡胶草 愈伤组织 再生体系 

分 类 号：S567[农业科学—中草药栽培]