低温胁迫对木薯组培苗叶片若干代谢生理指标的影响  

Effects of Low Temperature Treatment on the Physiological Characteristics of Cassava Tissue Culture Seedling Leaves

作　　者：欧文军 罗秀芹[2] 李开绵[2] 

机构地区：[1]海南大学农学院,海南海口570228 [2]中国热带农业科学院热带作物品种资源研究所农业部木薯种质资源保护与利用重点实验室,海南儋州571737

出　　处：《热带作物学报》2015年第4期706-712,共7页Chinese Journal of Tropical Crops

基　　金：中南美洲热带作物种质资源收集与合作研究(No.2011DFB31690);重要热带作物优异和特色种质资源的引进[No.2011-G13(2)]

摘　　要：以木薯SC8和MS5供试材料,研究5℃低温处理0、7、10、12和15 d对木薯组培苗叶片若干生理代谢指标的影响。结果表明:木薯种质SC8和MS5在5℃低温胁迫下,随着胁迫时间的延长,叶片的相对电导率、可溶性糖、丙二醛含量呈不断上升趋势,而可溶性蛋白、脯氨酸含量、超氧化物歧化酶活性、过氧化物酶活性呈先上升后下降的变化趋势;低温胁迫下,叶片的相对电导率与丙二醛含量和可溶性糖含量呈极显著正相关,与超氧化物歧化酶活性和可溶性蛋白含量呈极显著负相关;可溶性蛋白含量与丙二醛含量呈显著负相关,与游离脯氨酸含量、超氧化物歧化酶活性、过氧化物酶活性呈极显著正相关;超氧化物歧化酶活性与可溶性糖含量呈极显著负相关,与过氧化物酶活性呈极显著正相关;主成分分析显示超氧化物歧化酶活性、可溶性蛋白、可溶性糖和相对电导率在第1主成分中占有最大的信息量,与木薯耐寒性关系密切,可作为评价木薯耐寒性强弱的主要生理指标,其次是可溶性蛋白、丙二醛含量和过氧化物酶活性。综合分析生理数据认为木薯MS5的耐寒性高于SC8,这为下一步从离体保存库中直接采用组培苗方式进行大规模筛选耐寒种质提供参考方法。Cassava accessions SC8 and MS5 were taken as test materials. After with five different time（0, 7, 10, 12,15 d） of treatments at 5 ℃ low temperature stress, the relative conductivity, soluble protein content, soluble sugar content, proline content, MDA content, enzyme activity of SOD and POD were measured. The experimental result reveals that as the extension of stress time, cassava seedling leaves＇ relative conductivity rate, soluble sugar content and MDA content presented a constantly rising trend, while soluble protein content, proline content,enzyme activity of SOD and POD firstly increased and then decreased finally. Under cold stress, cassava relative conductivity rate was very significantly positively correlated to MDA and soluble sugar content, while very significantly negatively correlated to enzyme activity of SOD and soluble protein content. Soluble protein content was significantly negatively correlated to MDA content and very significantly positively correlated to proline content and enzyme activity of SOD and POD, and SOD activity was very significantly negatively correlated to soluble sugar content and very significantly positively correlated to POD activity. Principal component analysis indicates that SOD activity, soluble protein content, soluble sugar content and the relative conductivity account for the largest amount of information in the first principal components, closely related to cassava cold tolerance, which could be used as physio1 ogical in dices to identify cold resistance of cassava accessions or varieties. MS5 had much stronger cold-resistance than SC8 by using the Comprehensive analysis of biological data. The result provideda reference method for large-scale screening of cold resistant cassava germplasm by using tissue culture tube seedling directly.

关 键 词：木薯 叶片 低温胁迫 生理指标 

分 类 号：S537[农业科学—作物学]