叶用莴苣热激蛋白LsHsp70-3701和LsHsp70-1707基因与抽薹特性的表达分析  

Expression Analysis of Heat-shock-protein LsHsp70-3701 and LsHsp70-1707 Gene from Leaf Lettuce(Lactuca sativa L.) in Bolting

作　　者：赵真真[1] 韩莹琰[1] 范双喜[1] 李婷[1] 王振雨 刘秀萍 

机构地区：[1]北京农学院,北京昌平102206 [2]北京市优质农产品产销服务站,北京朝阳100101

出　　处：《安徽农业科学》2015年第14期27-29,共3页Journal of Anhui Agricultural Sciences

基　　金：国家自然科学基金项目"叶用莴苣高温抽薹相关蛋白的鉴定及其互作分析"(31372057);国家自然科学基金项目"叶用莴苣高温胁迫响应因子LsHsp70的功能研究"(31401883);北京市叶类蔬菜创新团队建设专项(blvt-02);北京市农业科技项目"生菜耐热新品种繁育及安全生产技术试验示范"(20140134);北京市科技成果转化提升计划项目"高产优质生菜产业化关键技术示范与成果转化"(PXM2015_014207_000012)

摘　　要：[目的]探讨叶用莴苣热激蛋白Ls Hsp70-3701和Ls Hsp70-1707基因在抽薹前后的表达水平,分析Hsp70基因与叶用莴苣抽薹的相关性。[方法]采用实时荧光定量方法检测Ls Hsp70-3701和Ls Hsp70-1707基因在不同品种中的相对表达量,分析其差异性。[结果]Ls Hsp70-3701基因在热敏品种P-S11抽薹期的表达量显著高于抽薹前;在耐热品种G-S59抽薹初期的表达量与抽薹前无显著差异,但后期表达量显著降低。Ls Hsp70-1707基因在热敏品种P-S11抽薹初期表达量与抽薹前差异不显著,但其后表达水平显著高于抽薹前;在GS59中抽薹期的最大表达量低于抽薹前的最大表达量,但两者差异不显著,而其他时间的相对表达量却显著低于抽薹前最高水平。[结论]Ls Hsp70-3701和Ls Hsp70-1707基因与叶用莴苣抽薹具有一定的相关性,叶用莴苣抽薹是差异基因表达的结果。[Objective] The research preliminarily studied the expression of heat-shock-protein LsHsp70-3701 and LsHsp70-1707 and analyzed the correlation between Hsp70 gene and belting of leaf lettuce. [ Method] Real-time quantitative PCR （qRT-PCR） was used to analyze the LsH- sp70-3701 and LsHsp70-1707 expression differences in different varieties of Lactuca sativa. [ Result] The LsHsp70-3701 gene expression level in bolting were significantly higher than that before belting in P-S11. The expression of the early belting was no significant difference with the former beltingthe expression and significantly decreased in the late in G-S59. The difference of LsHsp70-1707 gene expression inearly belting and before bolting was not significant,but then expression levels were significantly higher than before belting. The maximum expression of belting lower than the maximum expression of before belting and the difference was not significant in G-S59, but the expression of the other time was significantly lower than the highest level of before belting. [ Conclusion] LsHsp70-3701 and LsHsp70-1707 have some relevance with belting of leaf lettuce,and belting is the result of differences in gene expression.

关 键 词：叶用莴苣 HSP70 抽薹 表达分析 

分 类 号：S188[农业科学—农业基础科学]