肺表面活性蛋白作为矽肺患者早期生物标志物的研究  

Early biomarkers of silicosis: pulmonary surfactant protein

作　　者：闫立成[1] 曹福源[2] 赵海鹰[3] 翟瑞雪[1] 姚林[2] 张艳淑[1] 

机构地区：[1]河北联合大学公共卫生学院,河北省煤矿卫生与安全实验室,河北唐山063009 [2]河北联合大学医学实验动物中心,河北唐山063009 [3]中国人民解放军第255医院,河北唐山063000

出　　处：《现代预防医学》2015年第9期1562-1563,1566,共3页Modern Preventive Medicine

基　　金：河北省科技支撑项目(09276194D)

摘　　要：目的探讨肺表面活性蛋白(PS)作为矽肺早期生物标志物的可能性,为矽肺的早期诊断和临床治疗提供理论依据。方法 87名矽尘作业工人,按照接尘工龄分为大于10年组和小于以及等于10年组。应用酶联免疫分析法(ELISA)检测矽尘作业工人血清中SP-A、SP-B、SP-C、SP-D的蛋白含量。用斯皮尔曼分析方法确定PS和接尘工龄的相关性。结果矽尘作业工人血清中SP-A、SP-B、SP-C、SP-D含量随着接尘工龄的增加而增加。其中SP-A、SP-C和SP-D含量均与接尘工龄呈正相关。结论矽尘作业工人血清中SP含量发生改变,其中SP-C的水平可作为矽肺的早期效应标志物。Objective To explore the possibility of the pulmonary surfactant protein as the early biomarkers of silicosis, so as to provide evidences for the early diagnosis and therapy of silicosis. Methods Firstly, we divided 87 silica-exposed workers into two groups： one group that the working-age of dust exposure is above 10 years and the other group that the working-age of dust exposure is below 10 years respectively. Secondly, we detected the concentrations of SP-A, SP-B, SP-C and SP-D in serum by ELISA. Meanwhile, we analyzed the correlation between PS and the working-age of dust exposure by spearman＇s correlation. Results The concentrations of SP-A, SP-C and SP-D in serum of silica-exposed workers increased with the increase of the working-age of dust exposure. And the contents of SP-A, SP-C and SP-D had positive correlations with the working-age of dust exposure. Conclusion The content of SP in serum of the silica-exposure workers changes, and the level of SP-C can be served as an early biomarker of silicosis.

关 键 词：矽肺 矽尘作业工人 肺表面活性蛋白 效应标志物 

分 类 号：R13[医药卫生—劳动卫生]