基于双光子共聚焦成像的急性高眼压大鼠小梁网孔隙率变化研究  

Change of porosity in trabecular meshwork under acute high intraocular pressure using two-photon microscope

作　　者：任琳[1,2] 梅曦[1,2] 刘志成[1,2] 

机构地区：[1]首都医科大学生物医学工程学院 [2]首都医科大学临床生物力学应用基础研究北京市重点实验室,北京100069

出　　处：《北京生物医学工程》2015年第2期181-184,共4页Beijing Biomedical Engineering

基　　金：国家自然科学基金(31070840);北京市自然科学基金(3122010);首都医科大学重点实验室开放课题项目(2014LCSW01)资助

摘　　要：目的获取不同眼压下小梁网组织深层结构信息,为小梁网房水外排通道生理功能的探索奠定组织形态学基础。方法将4只SD大鼠分成A、B两组每组2只,处死后于左眼球分别加压40mm Hg(A组)、加压60 mm Hg(B组),维持24 h。右眼均为未加压对照组,利用双光子共聚焦成像系统采集每只眼球的小梁网组织形态图:从眼底剖开眼球后照射前房角小梁网处,每2μm采集图像,直至图像模糊停止。结果未加压的对照组眼球小梁网处胶原纤维排列较为规则,孔隙明显,小梁网与周围组织界限分明。加压40 mm Hg的A组眼球小梁网胶原纤维出现了部分塌缩,小梁网与周围组织出现融合,偶尔可见一些孔隙,胶原纤维排列呈无序状态。加压60 mm Hg的B组眼球小梁网胶原纤维断裂较明显,临管区被挤压到完全塌陷,与周围组织已无法分辨。A、B两组动物的小梁网均表现出骨架断裂,组织变薄,逐渐与周围组织融为一体,以及出现远端的巩膜静脉塌缩的现象。与A组眼球相比,B组眼球的葡萄膜小梁网孔隙率略有增加。结论急性眼内压升高可能引发房水外排通道结构异常,主要表现为前房角小梁网组织压缩、巩膜静脉塌陷。这一解剖结构异常造成房水排出困难,从而又加剧了眼内压的升高。Objective To obtaln morphological change of trabecular meshwork under different pressure and do certaln basic research for exploring aqueous humor discharge. Methods Four SD rats were divided into A group and B group. Enucleated left eyes were perfused at pressure of 40 mm Hg（A group）and 60mmHg（B group）for 24 hours to achieve high IOP and right eyes were control group. We used two-photon confocal system to observe morphological images of trabecular meshwork in each eye：to split from fundus and expose the limbus,then we got the images each 2μm until fuzziness. Results The collagen fiber in trabecular meshwork of the control group arranged regularly. We could see obvious boundaries between trabecular meshwork and surrounding tissue. After perfused 40 mmHg pressure,the collagen fiber of trabecular meshwork became collapsed and merged with surrounding tissue. The fibers arranged in disorder. After＆amp;nbsp;perfused 60 mmHg,collagen fiber of trabecular meshwork fractured more obviously,juxtacanalicular connective tissue collapsed completely,and difficult to be distinguished from the surrounding tissue. Conclusions Acute intraocular pressure might cause the structure abnormal in aqueous humor discharge channel：trabecular meshwork tissue in anterior chamber became compressed,and sclera vein collapsed. This abnormal anatomy caused excreted difficulty of aqueous humor,and aggravated the elevated intraocular pressure in return.

关 键 词：小梁网 高眼压 孔隙率 巩膜静脉 双光子共聚焦成像系统 

分 类 号：R318.04[医药卫生—生物医学工程]