HIV感染者脑脊液淋巴细胞亚群分析  

Analysis of the lymphocyte subsets in cerebrospinal fluid in patients with HIV infection

作　　者：乔曦[1] 刘毓刚[1] 李艳梅[1] 沈金兰[1] 曾平[1] 刘彤[1] 王艳艳[1] 

机构地区：[1]成都军区总医院实验医学中心生化科/高湿医学全军重点实验室,四川成都610083

出　　处：《国际检验医学杂志》2015年第8期1052-1054,共3页International Journal of Laboratory Medicine

摘　　要：目的了解HIV感染者脑脊液淋巴细胞亚群分布,探讨脑脊液淋巴细胞亚群在HIV中枢神经系统并发症发生中的意义。方法选取HIV感染者34例,其中20例无神经系统症状(单纯HIV组),14例伴有神经系统症状(神经系统HIV组),另选取15例健康人(健康对照组)。使用流式细胞术检测脑脊液淋巴细胞亚群,采用免疫比浊法检测IgG水平,并进行比较分析。结果单纯HIV组和神经系统HIV组脑脊液CD8^+T淋巴细胞百分率高于健康对照组,CD4^+T淋巴细胞百分率低于健康对照组,HIV感染者IgG水平高于健康对照组,差异均有统计学意义(P<0.01);而B淋巴细胞、NK细胞百分率差异均无统计学意义(P>0.05);单纯HIV组和神经系统HIV组间脑脊液各淋巴细胞亚群百分率差异无统计学意义(P>0.05)。结论 HIV感染者在早期未发生神经系统并发症时就出现脑脊液免疫功能紊乱,淋巴细胞亚群变化趋势与外周血中一致,T淋巴细胞亚群可能与HIV神经系统症状相关。Objective To explore the lymphcyte subsets distribution in cerebrospinal fluid in patients with HIV infection ,and to investigate the clinical significance of the lymphocyte subsets in cerebrospinal fluid in HIV central nervous system complication . Methods 34 patients with HIV infection ,including 20 patients without nervous system symptoms （simple HIV group） and 14 patients with nervous system symptoms （neurological HIV group） ,and 15 cases of healthy people （control group） were selected . Flow cytometry was used to detect lymphocyte subsets ,and immunoturbidimetry was used to detect the level of IgG in cerebrospinal fluid .Results The percentage of CD8^＋ T cells was higher and percentage of CD4^＋ T cells was lower in the simple HIV group and neurological HIV group than those in the healthy control ,with statistically significant differences （P〈0 .01） .The level of IgG in patients with HIV infection was higher than that in the healthy control group （P〈0 .01） .While no significant difference were found in the percentage of B cells and NK cells among the there group （P〉0 .05） .There were also no significant differece between the simple HIV group and neurological HIV group in the ratio of each lymphcyte subset in cerebrospinal fluid （P〉0 .05） .Conclusion The immune disorder in cerebrospinal fluid in patients with HIV infection may appear in the early time before the nervous system complication .The changing trends of lymphocyte subsets are consistent with the peripheral blood ,which demonstrate that the T lymphocyte subsets may be correlated with the nervous system symptoms of HIV .

关 键 词：人类免疫缺陷病毒 淋巴细胞亚群 流式细胞术 脑脊液 

分 类 号：R512.91[医药卫生—内科学] R446.14[医药卫生—临床医学]