抗凝剂对血小板计数结果的影响分析  

Influence of anticoagulants on platelet count

作　　者：黄亮[1] 梁瑜金 

机构地区：[1]肇庆市第二人民医院检验科,广东肇庆526060

出　　处：《国际检验医学杂志》2015年第8期1104-1105,共2页International Journal of Laboratory Medicine

摘　　要：目的分析不同抗凝剂对血小板计数结果的影响。方法选择5例乙二胺四乙酸二钾(EDTA-K2)抗凝全血标本血细胞分析仪计数血小板结果重度减低的患者,采集EDTA-K2、枸橼酸钠、肝素锂、氟化钠抗凝静脉血标本和手指末梢血标本,分别采用血细胞分析仪(仪器法)和显微镜计数法(手工法)计数血小板,并同时制备涂片标本,经瑞氏-姬姆萨染液染色后显微镜镜检。结果 5例患者EDTA-K2、枸橼酸钠、肝素锂及氟化钠抗凝静脉血标本仪器法、手工法血小板计数结果均明显低于手指末梢血标本,血涂片标本镜检均可见血小板大片聚集;手指末梢血标本仪器法、手工法血小板计数结果均正常,血涂片标本镜检可见血小板分布正常,无聚集现象。结论多种抗凝剂均有可能诱导血小板发生聚集,从而引起血小板假性减少。发生抗凝剂依赖性血小板假性减少时,应采用不加抗凝剂的手指末梢血标本进行血小板计数,从而获得准确结果。Objective To analyze the influence of anticoagulants on platelet count .Methods A total of 5 patients with decreased platelet level ,detected by using haemocyte analyzer and samples anticoagulated by ethylene diamine tetraacetic acid dipotassium （EDTA‐K2 ） ,were enrolled ,and EDTA‐K2 ,sodium citrate ,heparin lithium ,sodium fluoride anticoagulated venous blood samples and fingers peripheral blood samples were collected and detected for platelet by using haemocyte analyzer and microscopic detection . Wright‐Giemsa′s stained cell smears were prepared and observed by using microscope .Results Platelet levels of anticoagulated samples ,detected by haemocyte analyzer and microscopic detection were obviously decreased ,and with large platelet aggregation ob‐served under microscope .Platelet levels of finger peripheral blood samples detected by haemocyte analyzer and microscopic detection were normal ,and without platelet aggregation observed under microscope .Conclusion Various anticoagulants could cause pseudo‐thrombocytopenia ,and fingers peripheral blood samples ,without anticoagulants ,should be used for platelet count to obtain accurate results .

关 键 词：乙二胺四乙酸二钾 枸橼酸钠 氟化钠 肝素锂 血小板假性减少 

分 类 号：R446.1[医药卫生—诊断学]