蜜蜂残翼病病毒PCR检测及5′-UTR基因序列分析  

PCR Detection of Deformed Wing Virus and Sequence Analysis of 5′-UTR Gene

作　　者：郑言[1] 宋战昀[2] 杨倩[1] 王向辉[1] 隋佳辰 张健[1] 王振国[2] 李敏思[3] 

机构地区：[1]吉林农业大学,吉林长春130118 [2]吉林出入境检验检疫局,吉林长春130062 [3]甘肃农业大学,甘肃兰州730070

出　　处：《动物医学进展》2015年第5期52-55,共4页Progress In Veterinary Medicine

基　　金：国家自然科学基金项目(31001065);质检总局科技项目(2013IK033)

摘　　要：从吉林省某蜂场疑似蜜蜂残翼病病料中分离到一株蜜蜂残翼病病毒（DWV）,命名为DWVJL1,提取该分离株的总RNA,采用RT-PCR技术扩增其基因组的5′-UTR并测序。测序结果与GenBank公布道的DWV 5′-UTR序列同源性在95%~99%之间。将测序结果与GenBank上公布的9个相关序列进行系统遗传进化树分析,证实其与JX878304同源性最近,而与GU109335、KJ437447同源性最远。A strain of deformed wing virus was isolated from suspected deformed wing virus disease materi- als in an apiary of Jilin and was named DWV-JL1. Total RNA was extracted from the isolate and a frag- ment of 5＇-UTR gene was amplified by RT-PCR. The sequencing results showed that 5＇-UTR had high ho- mology about 95 %-99 % compared with the report in GenBank. According to the phylogenetic tree based on 5＇-UTR,we confirmed that it had the highest homology with JX878304 and the lowest homologies with GU109335 and KJ437447.

关 键 词：蜜蜂残翼病病毒 5′-UTR 克隆和测序分析 

分 类 号：S895.9[农业科学—特种经济动物饲养]