一株鸽新城疫病毒的分离鉴定及F与HN基因序列分析  

Isolation,Identification and Sequence Analyses of F and HN Genes of One Newcastle Disease Virus Strain in Pigeons

作　　者：钟植文 黎先伟 陈珊 高绮静 刘镇明[2] 杨傲冰 

机构地区：[1]广东永顺生物制药股份有限公司,广东广州511356 [2]华南农业大学兽医学院,广东广州510642

出　　处：《动物医学进展》2015年第5期86-90,共5页Progress In Veterinary Medicine

摘　　要：从广东珠三角地区某鸽场的发病鸽群采样,接种10日龄SPF鸡胚后分离到一株病毒,命名为JP株。系列微量血凝和微量血凝抑制试验结果表明,新城疫病毒（NDV）阳性。测序结果显示,该NDV分离株F蛋白裂解位点的氨基酸序列为112 R-R-Q-K-R-F117,具有强毒株序列特点;F基因分型表明,JP株属于基因Ⅵ型。Blast结果显示,JP株F、HN基因序列均与基因Ⅵ型pi/CH/LGD/110208株同源性最高,均达99%。同源性比较发现,F和HN基因、蛋白都与基因Ⅵ型新城疫毒株同源性较高,分别为92.5%~99%、95.5%~99.1%和90.6%~98.7%、91.9%~99%,而与国内常用疫苗株B1、La Sota、Mukteswar等同源性较低,分别为83.2%~85.9%、89.2%~91%和79.3%~83.4%、86%~89.5%。分离的JP株属于基因Ⅵ型,并与国内常用疫苗株存在一定的差异。Some sick pigeons were sampled from pigeon farm in Pearl River Delta region, and used to inocu- late 10 day-old SPF chicken embryos, and a virus strain named JP was isolated. The positive result of Newcastle disease virus was determined by HA and HI tests. The result of sequence analysis showed that the amino-acid sequence of the fusion protein cleavage site was 112R-R-Q-K-R-F117, a typical sequence of virulent strains. Genetic analysis showed that the isolate belonged to genotype V[. The result of Blast showed that the sequences of F and HN genes of the isolate have the highest identities with the genotype ⅥNDV （pi/CH/LGD/110208）, both with 99%. The homology analyses of F and HN genes and proteins showed that the isolate were high identities with the genotype VI NDV, with 92.5%-99%, 95.5%-99.1% and 90.6%-98.7%, 91.9%-99%, respectively, but low identities with the common vaccine strains, such as B1, Mukteswar and La Sota, only with 83.2%-85.9%, 89.2%-91% and 79.3%-83.4%, 86%-89.5%, respectively, the isolate differs from the common vaccine strains.

关 键 词：鸽 新城疫病毒 F基因 HN基因 基因Ⅵ型 

分 类 号：S852.659.5[农业科学—基础兽医学]