香蕉细菌性枯萎病菌超分支滚环扩增快速检测技术  

Rapid detection for Ralstonia solanacearum race 2 using hyperbranched rolling circle amplification

作　　者：王念武 陈劲松 沈建国[3] 黄振 翁瑞泉 

机构地区：[1]福清出入境检验检疫局,福建福清350300 [2]泉州出入境检验检疫局,福建泉州362000 [3]福建出入境检验检疫局,福建福州350002 [4]福州机场出入境检验检疫局,福建福州350002

出　　处：《亚热带农业研究》2015年第1期1-5,共5页Subtropical Agriculture Research

基　　金：福建省科技厅资助项目(2012N0001)

摘　　要：根据香蕉细菌性枯萎病菌(Ralstonia solanacearum race 2)的特异插入序列ISRso19设计滚环扩增的锁式探针,并对超分支滚环扩增的反应条件进行优化,建立了香蕉细菌性枯萎病菌超分支滚环扩增技术。结果表明,该检测技术能够从供试的9种病原菌中特异性地检测出香蕉细菌性枯萎病菌,检测的DNA最低浓度为500 fg·μL-1,高于常规PCR的灵敏度。A padlock probe was designed based on the sequence of insertion sequence ISRso19 of Ralstonia solanacearum race 2 （ Rs2 ） , according to the design principle of padlock probe and primers. Detection system of hyperbranched rolling circle amplifica- tion （HRCA） was established and optimized. Results showed that Rs2 could be detected specifically by this method, while other reference plant pathogens could not be detected. The detection sensitivity of HRCA for DNA concentration was about 500 fg · μL-1, higher than conventional PCR method.

关 键 词：香蕉细菌性枯萎病菌 超分支滚环扩增 锁式探针 

分 类 号：S41-30[农业科学—植物保护]