经阴道注入重组白细胞介素17对正常妊娠CBA/J孕鼠的影响  

The Effect of Recombinant IL-17 Administered in the Vaginal Vaults on Normal Pregnancy CBA/J Mice

作　　者：柳红杰[1] 曲洪美[1] 马宏仲[2] 殷伟红[1] 于丽丽[1] 周小娇[3] 王文娟[3] 

机构地区：[1]青岛大学医学院附属烟台毓璜顶医院产科,山东省烟台市264000 [2]青岛大学医学院附属烟台毓璜顶医院麻醉科,山东省烟台市264000 [3]青岛大学医学院附属烟台毓璜顶医院生殖医学中心,山东省烟台市264000

出　　处：《国际妇产科学杂志》2015年第2期220-222,共3页Journal of International Obstetrics and Gynecology

基　　金：国家自然科学基金青年面上项目(81370013);国家自然科学基金青年项目(81000277)

摘　　要：目的：观察经阴道注入重组白细胞介素17（r IL-17）对正常妊娠CBA/J孕鼠（CBA/J雌鼠×BALB/c雄鼠）妊娠结局的影响,探讨其在诱导母胎免疫排斥中的作用。方法：将6-8周龄清洁级CBA/J（雌）和BALB/c（雄）近交系小鼠分为3组。对照组：正常妊娠模型CBA/J孕鼠未作任何干扰。磷酸盐缓冲液（PBS）组：正常妊娠模型CBA/J孕鼠,于受孕第1天麻醉状态下经阴道注射50μL PBS。r IL-17组：正常妊娠模型CBA/J孕鼠,于受孕第1天麻醉状态下经阴道注射50μL r IL-17。受孕后第14天观察胚胎丢失率。通过实时荧光定量聚合酶链反应（q RT-PCR）方法、蛋白质印迹（Western blotting）方法检测鼠蜕膜转化生长因子β（TGF-β）和IL-10的表达。结果：给予正常妊娠模型孕鼠外源性r IL-17,能引起其胚胎丢失率明显增加,且蜕膜TGF-β和IL-10 m RNA及蛋白的表达均明显降低（P〈0.05）。而对照组与PBS组相比,胚胎丢失率及TGF-β和IL-10 m RNA及蛋白的表达差异均无统计学意义（均P〉0.05）。结论：经阴道注入r IL-17能够引起正常妊娠模型CBA/J孕鼠胚胎丢失率增加,并引起母胎界面免疫耐受细胞因子分泌异常。Objective： To investigate the effect of transvaginal injected recombinant IL-17 on the pregnancy outcome in the CBA/J mice model （CBA/JxBALB/c） and its role in feto-maternal immune rejection. Methods：Six to eight-week-old female CBA/J and male BALB/c were divided into three different experimental groups. Mice of normal pregnancy （CBA/Jx BALB/c ） received no treatment were served as control group. 50 μL phosphate-buffered saline （PBS） or 50 p.L recombinant IL-17 （rIL-17） was administered in the vaginal vaults of anesthetized pregnant CBA/J mice on Day 1 of pregnancy served as PBS group and rIL-17 group individually. The numbers of surviving and reabsorbed fetuses in each group were counted, and the expression of TGF-β and IL-IO in decidua was assessed by quantitative real-time PCR and western blotting on Day 14 of pregnancy. Results：In this in vivo model of normal pregnancy, exogenous rIL-17 resulted in increased spontaneous resorption of fetuses and decreased expression of TGF-β and IL-10 （P〈0.05）. And there was no statistical difference between PBS group and control group （P〉0.05）. Conclusions ：Intravaginal rIL-17 can induce inflammation in fetomaternal interface resulting in abortion in normal pregnant CBA/J mice model.

关 键 词：白细胞介素10 白细胞介素17 转化生长因子Β 妊娠 动物 

分 类 号：R714.1[医药卫生—妇产科学]