HBV X蛋白对阿霉素诱导的肝癌细胞凋亡的影响研究  

Hepatitis B virus X protein suppresses adriamycin-induced apoptosis of hepatocellular carcinoma cells and its possible mechanism

作　　者：范红梅[1] 杨林[2] 孙士敏[1] 别彩群[1] 钟志民[1] 

机构地区：[1]广州医科大学附属深圳沙井医院内一科,广东深圳518104 [2]中山大学附属第三医院感染病科,广东广州510630

出　　处：《齐齐哈尔医学院学报》2015年第11期1563-1566,共4页Journal of Qiqihar Medical University

摘　　要：目的探讨HBV X蛋白对阿霉素处理后的Hep G2细胞凋亡影响及可能机制。方法用阿霉素处理Hep G2、Hep G2/GFP、Hep G2/GFP-HBx细胞,分别用台盼兰染色和流式细胞仪检测各组细胞凋亡率;并通过RT-PCR和Western blot检测P53、PTEN的基因及蛋白表达。结果阿霉素处理36小时后,Hep G2/GFP-HBx细胞凋亡率明显低于另外两组(P<0.01);Hep G2/GFP-HBx细胞PTEN mRNA和蛋白水平均低于另外两组(P<0.01),而p53 mRNA和蛋白水平与另外两组无明显差别(P>0.05)。结论 HBV X蛋白能够抑制阿霉素诱导的Hep G2细胞凋亡和PTEN的表达,HBV X蛋白可能通过影响p53-PTEN环路而抑制细胞凋亡。Objective To investigate the effect of hepatitis B virus X protein in the Adriamycin-induced Apoptosis of HepG2 Cells and its possible mechanism.Methods HepG2, HepG2/GFP and HepG2/GFP-HBx cells were treated with adriamycin, and apoptotic cell death were examined by trypan blue exclusion and flow cytometry analysis.The transcription of p53, PTEN was analyzed by RT-PCR, and the expression of p53, PTEN was analyzed by western blot.Results After treated with adriamycin 36 hours,the apoptosis rate of HepG2/GFP-HBx was significantly lower than HepG2 cells and HepG2/GFP cells;the transcription and expression of PTEN in HepG2/GFP-HBx cells decreased, but there was no significant change in the transcription and expression of p53 in HepG2/GFP-HBx cells.Conclusions HBV X protein inhibited apoptosis of HepG2 cells induced by adriamycin and PTEN expression.The inhibitory effect of HBV X protein on the cell apoptosis may be related to the inhibition of p53-PTEN pathway.

关 键 词：HBV X蛋白 阿霉素 HEPG2细胞 细胞凋亡 PTEN P53 

分 类 号：R282.71[医药卫生—中药学]