疏肝和胃汤对抑郁模型大鼠延髓、脊髓及胃粘膜组织血管活性肠肽表达的影响  

Effect of Shugan Hewei Decoction on the Expression of VIP in Depression Model Rats of Brain、Spinal Cord and Gastric Mucosa

作　　者：刘松林[1] 岳滢滢[1] 邢颖[1] 李明珠[1] 李家庚[1] 程方平[1] 

机构地区：[1]湖北中医药大学中医临床学院,湖北武汉430065

出　　处：《时珍国医国药》2015年第4期777-779,共3页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金项目(No.81072720);教育部新世纪优秀人才支持计划资助项目(No.NCET-11-0963)

摘　　要：目的观察疏肝和胃汤对抑郁模型大鼠延髓、脊髓及胃粘膜组织血管活性肠肽（VIP）表达的影响,探讨疏肝和胃汤改善抑郁状态及胃肠功能的作用机制。方法 100只大鼠随机分成生理盐水组、模型组、百优解组及疏肝和胃汤大、小剂量组。采用慢性心理应激加孤养法制作抑郁模型,共计造模4周。在第3周,百优解组及疏肝和胃汤大、小剂量组分别予以0.36mg/（kg·d）、20g/（kg·d）、10g/（kg·d）体重给药,生理盐水组及模型组给予等体积生理盐水,每天分2次灌胃。在第4周结束后,使用内固定法取大鼠延髓迷走神经背核部位的脑、胸髓（T6-T8,根据脊神经定位）及胃粘膜组织,应用免疫组织化学技术检测上述部位VIP的表达。结果模型组大鼠延髓、脊髓中VIP阳性表达明显下降,胃粘膜VIP阳性表达明显上升,与生理盐水组比较有显著性差异（P〈0.05或P〈0.01）;治疗2周后,疏肝和胃汤大、小剂量组和百优解组大鼠延髓、脊髓组织中VIP阳性表达显著上升,胃粘膜VIP阳性表达明显下降,与模型组比较有显著性差异（P〈0.05或P〈0.01）;其中,疏肝和胃汤大剂量组与百优解组延髓中VIP含量比较无显著性差异（P〉0.05）,但脊髓与胃粘膜中VIP含量与百优解组相比,有显著性差异（P〈0.05）。结论疏肝和胃汤可能是通过增加延髓、脊髓中VIP的表达,降低胃黏膜中VIP的表达,双向调节＂脑（延髓）-脊髓-胃＂脑肠轴通路中VIP的含量,达到改善抑郁状态与胃肠功能的作用。Objective To observe the effect of Shugan Hewei Decoction （SHD） on the expression of vasoactive intestinal peptide （VIP） in depression model rats of brain（ medulla oblongata）, spinal cord and gastric mucosa , to research the possible mechanism of SHD to relieve depression and improve gastrointestinal function. Methods Totally 100 wistar rats were randomized into the normal saline group, model group, Fluoxetine group and the high, low dosage of SHD groups, with 20 in each. The depression models were established with Chronic Unpredictable Mild Stress （CUMS） and separation according to related references for 4 weeks. At the 3rd week, the model each treatment group began to medicine. Fluoxetine group and high, low dosage of SHD group and were given Fluoxetine and high, low dosage of SHD by 0.36mg/（ kg · d）,20g/（ kg · d） and 10g/（ kg·d） in weights through introgastric administration respectively, twice a day ; the normal saline group and model group were given the same volume physiological saline. Every group must be given continuous course treatment for fourteen days. At the end of the fourth week, the internal fixation method was used to take brain（ medulla oblongata vagus nerve nucleus） tissue ,spinal cord tissue （T6 -T8 ） and gastric mucosa tissue. The method of immunohistochemistry （IHC） was used to detect the expression of VIP in the above organizations. Results Comparing with the normal saline group, the content of VIP decreased obviously in brain （medulla oblongata） and spinal cord of model rats （ P 〈 0.01 ）, the content of VIP increased significantly in gastric mucosa（ P 〈 0.01 ）. Comparing with the model group, the content of VIP raised obviously in brain （medulla oblongata） and spinal cord of high, low dosage of SHD group and Fluoxetine group（ P 〈 0.05 or P 〈 0.01 ） , the content of VIP reduced significantly in gastric mucosa（ P 〈 O. 05 or P 〈 0. 01 ）. In addition, the effect of high dosage of SHD was equal to Fluoxetine group in

关 键 词：抑郁症 延髓 脊髓 胃粘膜 疏肝和胃汤 血管活性肠肽 

分 类 号：R285.5[医药卫生—中药学]