左归降糖解郁方对糖尿病并发抑郁症模型大鼠学习记忆及海马超微结构的影响  

Effects of Zuogui Jiangtang Jieyu Formulation on the Learning and Memory Abilities and Ultrastructure of Hippocampus in Diabetes Mellitus Rats with Depression

作　　者：张秀丽 王宇红 杨蕙[2] 孟盼 谭小雯 柴上 

机构地区：[1]湖南省中药粉体与创新药物省部共建国家重点实验室培育基地,湖南长沙410007 [2]湖南中医药大学第一附属医院,湖南长沙410007

出　　处：《时珍国医国药》2015年第4期787-789,共3页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金(No.81373578);湖南省自然科学基金(No.13JJ5030);湖南省科技厅计划项目(No.2012SK3141);湖南省高校创新平台开放基金项目(No.13k074)

摘　　要：目的研究左归降糖解郁方对糖尿病并发抑郁症大鼠学习记忆和海马超微结构的影响。方法采用高脂灌胃、链脲佐菌素注射(38mg/kg)和慢性应激联合的方法建立糖尿病并发抑郁症动物模型,并随机分为5组:糖尿病并发抑郁症模型组,阳性药(二甲双胍0.18g/kg+氟西汀1.8mg/kg)组,左归降糖解郁方高、中、低剂量(20.53 g/kg,10.26 g/kg,5.13g/kg)组,以正常组为对照。给药4周后,采用Morris水迷宫进行行为学评价,采用透射电镜观察海马细胞超微结构。结果Morris水迷宫实验,与正常组比较,模型组大鼠逃避潜伏期(EL)明显延长,空间探索时间(SET)明显缩短(P<0.05);与模型组比较,阳性药组和高剂量组大鼠EL明显缩短,SET明显延长(P<0.05)。电镜下可见,正常组大鼠海马神经元细胞形态规则,染色质分布均匀,线粒体内部嵴清晰可见;模型组大鼠海马神经元胞体肿胀,内质网脱颗粒严重,线粒体内嵴消失;给予左归降糖解郁方高剂量后,海马神经元损伤程度减轻,部分胞内可见线粒体。结论左归降糖解郁方可明显改善模型大鼠的学习记忆功能,其可能通过保护海马神经元结构发挥治疗作用。Objective To study the influence of Zuogui Jiangtang Jieyu formulation on the special learning and memory in diabetes mellitus with depression model rats. Methods Diabetes mellitus with depression rat models were established by high -fat diet, injection of streptozotocin （38mg/kg） and chronic stress. The model rats were randomly divided into five groups, the diabetes mellitus with depression groups, metformin（0.18g/kg） combined fluoxetine（1.8mg/kg） group, Zuogui Jiangtang Jieyu Formulation high, medium and low dose（2.28g/ml, 1.14g/ml,0. 57g/m1）group, and the SD rats were assigned into the control group. After 4 weeks of administration, Morris water maze was used to evaluate the cognitive function of rats and transmission electron microscope（TEM） was used to observed the changes of hippocampus ultrastructure. Results Compared with the normal group, the escape lateneies were significantly longer in the Morris water maze, as well as the space exploration times were significantly shortened in model group（ P 〈 0. 05 ） , and compared with the model group, the learning and memory capacity to be a certain degree of recovery in positive drug group and Zuogui Jiangtang Jieyu Formulation high dose group. TEM showed that the structure of hippocampus neutrons and neuropils were normal in control group. In rats of model group, hippocampal neurons were less, and mitochondria within the cytoplasm was swelling with the ridges disappeared. In Zuogui Jiangtang Jieyu Formulation high dose group, the above mentioned changes were ameliorated compared with model group. Conclusion The cognitive function could be improved by Zuogui Jiangtang Jieyu Formulation. Its function might be correlated with protecting the ultra - structure of hippocampal neurons.

关 键 词：糖尿病并发抑郁症 左归降糖解郁方 海马 超微结构 学习记忆 

分 类 号：R285.5[医药卫生—中药学]