血瘀型动脉硬化闭塞症血清代谢物和炎症反应相关因子的变化及意义  

Changes and Significance of Blood Stasis Type of Arteriosclerosis Obliteransassociated Factors in Serum Metabolites and Inflammatory Reaction

作　　者：李鑫[1,2] 李大勇[2] 吕延伟 李世征[2] 宋姗姗[2] 侯俊杰[2] 

机构地区：[1]辽宁中医药大学,辽宁沈阳110032 [2]辽宁中医药大学附属医院,辽宁沈阳110032

出　　处：《时珍国医国药》2015年第4期996-998,共3页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金(No.81173273);辽宁省自然科学基金(No.201202154);辽宁省高等学校优秀人才支持计划(No.LJQ2012083);辽宁省沈阳市科学技术项目(No.F12-277-1-73)

摘　　要：目的探讨血瘀型动脉硬化闭塞症(ASO)血清中代谢物和炎症反应相关因子的变化及意义。方法血瘀型ASO患者15例,健康对照10例,酶联免疫吸附(ELISA)双抗体夹心法检测血清中VEGF、TGF-β1和IL-1β水平,基于气相色谱-质谱联用技术(GC/MS)的代谢组学方法分析血清中内源性代谢物的变化。结果与对照组比较,ASO组血清中血清中IL-1β的含量明显升高,差异有极显著性意义(P<0.01);VEGF、TGF-β1含量轻度升高,差异无显著性意义;ASO组血清中的糖类、氨基酸类、脂肪酸类物质的代谢水平均发生了明显的改变。结论炎症反应和血清内源性代谢物的变化与血瘀型ASO的发病密切相关。Objective To study the changes and significance of blood stasis type of arteriosclerosis obliterans （ASO） of related factors in serum metabolites and inflammatory reaction. Methods 15 cases of patients with blood stasis type ASO, 10 healthy control subjects enzyme linked immunosorbent assay （ELISA） double antibody sandwich methodin detection of serum VEGF, TGF- β1 and IL- 1β level, gas chromatography -mass spectrometry （GC/MS） technique based on metabonomic analysis of endogenous metabolites in serum in the methodological change. Results Compared with the control group, the content of serum IL - 1 increased significantly in the serum in ASO group, there were very significant difference （P 〈 0. 01 ） ; VEGF, TGF - β1 levels increased slightly, and there were no significant differences in the metabolism of carbohydrates, amino acids; water, fatty acids in serum in ASO group of average had obvious changes. Conclusion Changes in the incidence of type ASO blood stasis and inflammation＇ and serum endogenous metabolites have close relationship.

关 键 词：动脉硬化闭塞症 血清代谢物 炎症反应 

分 类 号：R259[医药卫生—中西医结合]