Ⅱ型胶原对T-2毒素诱导关节软骨损伤拮抗作用  

Protective effect of collagen-Ⅱ on articular cartilage damage induced by T-2 toxin in rats

作　　者：萨如拉[1] 王丽华[2] 

机构地区：[1]内蒙古医科大学卫生管理学院社会医学教研室,内蒙古呼和浩特150081 [2]哈尔滨医科大学

出　　处：《中国公共卫生》2015年第5期603-605,共3页Chinese Journal of Public Health

摘　　要：目的观察Ⅱ型胶原干预T-2毒素诱导大鼠关节软骨早期损伤的作用,为探讨关节软骨损伤的防治措施提供理论依据。方法 Wistar大鼠40只,随机分为4组,每组10只,对照组(常规成品颗粒料),T-2毒素组(含100 ng/kg T-2毒素饲料),Ⅱ型胶原0.5、5.0组(0.5、5.0 g/L)连续处理5个月,光镜下观察大鼠透明软骨组织病理学改变,用酶联免疫吸附试验检测大鼠血清Ⅱ型胶原羧基端端肽(CTX-Ⅱ)、软骨寡聚基质蛋白(COMP)及尿中吡啶啉(DPD)含量。结果光镜下T-2毒素组大鼠关节软骨细胞排列紊乱,软骨细胞变形、变性,可见大面积软骨细胞坏死,而低、高剂量Ⅱ型胶原组表现为软骨表面原纤维形成,表层软骨细胞肿胀变圆,扁平的软骨细胞减少,软骨细胞簇集等骨关节炎早期病理改变。对照组,T-2毒素组,高、低剂量Ⅱ型胶原组大鼠血清CTX-Ⅱ、COMP含量分别为(25.07±9.17)、(39.17±10.49)、(28.20±9.74)、(29.73±9.32)μg/L与(6.38±2.23)、(24.53±4.26)、(20.32±4.74)、(19.44±4.92)μg/L,大鼠尿液DPD含量分别为(4.14±1.06)、(4.33±3.43)、(5.72±3.89)、(4.23±2.90)μg/L。与对照组比较,模型组大鼠血清CTX-Ⅱ、COMP含量明显升高(均P<0.05),大鼠尿液DPD含量无明显改变;与模型组比较,Ⅱ型胶原组大鼠血清CTX-Ⅱ、COMP明显降低(均P<0.05),大鼠尿液DPD含量无明显变化。结论Ⅱ型胶原能拮抗T-2毒素所致软骨损伤作用,延缓关节软骨的破坏进程,其机制可能与降低大鼠血清中CTX-Ⅱ及COMP含量有关。Objective To observe protective effect of collagen-Ⅱ on articular cartilage damage induced by T-2toxin in rats,and to provide a theoretical basis for the control of articular cartilage damage. Methods Forty Wistar rats were randomly divided into four groups（ 10 rats in each group） ： blank group（ fed with standard rat chow）,T-2-toxin exposed group（ with T-2-toxin of 100 ng / kg）,collagen-II exposed group（ with collagen-II of 0. 5 and 5. 0 g / L）. After the treatment of 5 months,the articular cartilage was observed with light microscope and the content of serum C-telopeptide of type Ⅱ collagen（ CTX-Ⅱ） and cartilage oligomeric matrix protein（ COM P） and urinary deoxypyridinoline（ DPD）were determined with enzyme-linked immunosorbent assay（ ELISA） kits. Results Disarrangement,degeneration,and necrosis of articular chondrocytes were observed in the rats of T-2-toxin exposed group; early pathological changes of osteoarthritis（ fibril formation,sw elling of chondrocytes on the cartilage surface,and cluster of chondrocytes） were observed in the rats of the two collagen-II exposed groups. The levels of serum CTX-Ⅱ,COM P,and urinary DPD were 25. 07 ±9. 17,6. 38 ± 2. 23,and 4. 14 ± 1. 06 μg / L for the blank group,39. 17 ± 10. 49,24. 52 ± 4. 26,and 4. 33 ± 3. 43μg / L for T-2-toxin group,29. 73 ± 9. 32,19. 44 ± 4. 92,and 4. 23 ± 2. 90μg / L for 0. 5 g / L collagen-II group,and 33. 20 ± 9. 74,20. 32 ± 4. 74,and 5. 72 ± 3. 89 μg / L for 5. 0 g / L collagen-II group,repectively. Compared with the blank group,the levels of serum CTX-Ⅱ and COM P in the T-2-toxin exposed group increased markedly,with a signigicant difference（ P〈0. 05）,and the levels of urinary DPD did not increase significantly. Compared with the model group,the levels of serum CTX-Ⅱ and COM P in the two collagen-II exposed groups decreased markedly,with a signigicant difference（ P〈0. 05）,and the level of urinary DPD did not increase significantly. Conclusion Collagen-Ⅱ could effective

关 键 词：软骨 关节 Ⅱ型胶原 T-2毒素 

分 类 号：R114[医药卫生—卫生毒理学]