高糖对肾小管上皮细胞PTEN蛋白表达影响  

Effect of high-glucose on PTEN protein expression and its role in renal tubular epithelial cells of rats

作　　者：张昌志[1] 石明隽[1] 李霜[1] 王圆圆[1] 刘丽荣[1] 石春花[1] 严瑞[1] 郭兵[1] 

机构地区：[1]贵阳医学院病理生理学教研室,贵州贵阳550004

出　　处：《中国公共卫生》2015年第5期609-611,共3页Chinese Journal of Public Health

基　　金：国家自然科学基金(81360116);贵州省社会发展攻关项目(黔科合SY字【2012】3088号)

摘　　要：目的观察高糖条件下肾小管上皮细胞第10号染色体缺失的磷酸酶和张力蛋白同源基因(PTEN)蛋白的表达变化,探讨其在肾小管纤维化病变中的作用及可能机制。方法体外培养大鼠肾小管上皮细胞株(NRK52E),随机分为对照组和高糖组,每组分别设2、12、24、48 h 4个时间点进行动态观察;采用免疫荧光细胞化学染色检测E-钙黏素(E-cadherin)和α-平滑肌肌动蛋白(α-SMA)表达变化,Western blotting检测各组PTEN、磷酸化蛋白激酶B[p-Akt1(Ser473)]、E-caderin和α-SMA蛋白表达变化。结果与对照组比较,高糖组培养24、48 h时,PTEN、E-caherin蛋白表达量[分别为(1.15±0.13)、(1.10±0.13)和(1.23±0.11)、(1.22±0.11)]均减少(均P<0.05),而p-Akt1(Ser473)、α-SMA蛋白表达量[分别为(1.53±0.13)、(1.60±0.13)和(1.86±0.10)、(1.91±0.10)]均增多(均P<0.05);PTEN与E-cadherin蛋白表达量呈正相关(r=0.58,P<0.05),与α-SMA蛋白表达量呈负相关(r=-0.61,P<0.05)。结论高糖培养的肾小管上皮细胞中PTEN蛋白表达明显减少,可能通过PI3K/Akt信号通路参与了肾小管纤维化病变的发生发展过程。Objective To investigate protein expressions of phosphatase and tensin homologue deleted on chromosome 10（ PTEN） under high-glucose condition and to explore its role and possible mechanism in rat renal tubule fibrosis pathological changes. Methods The rat renal tubular epithelial cells（ NRK52E） were cultured in vitro and randomly divided into a normal and a high glucose group. Immunofluorescence cytochemistry staining was used to assess the protein expression of E-cadherin and smooth musle actin-α（ α-SM A） and Western blot was used to determinate the PTEN,phosphorylated AKT1（ p-Akt1）^（ Ser^473）,E-caderin,and α-SMA protein expression in the cells of different groups at 2,12,24,and 48 hours of the treatment. Results Compared with those of the normal glucose group,the protein expressions of PTEN and E-caherin significantly reduced（ 1. 15 ± 0. 13,1. 10 ± 0. 13 and 1. 23 ± 0. 11,1. 22 ± 0. 11）（ all P〈0. 05） and the expressions of p-Akt1^（ Ser^473）and α-SM A increased remarkably（ 1. 53 ± 0. 13,1. 60 ± 0. 13 and 1. 86 ± 0. 10,1. 91 ±0. 10）（ all P〈0. 05） in high glucose group at 24 and 48 hours of the treatment. The expression of PTEN was positively correlated with E-cadherin protein（ P〈0. 05） and negatively correlated with α-SM A expression（ P〈0. 05）. Conclusion The expression of PTEN protein significantly reduced in rat renal tubular epithelial cells 24 and 48 hours after high-glucose treatment and the decrease may facilitate the initiation and development of renal tubule fibrosis by alleviating the inhibitory effect through PI3 K / Akt signal pathw ay.

关 键 词：肾小管上皮细胞 高糖 磷酸酶和张力蛋白同源基因(PTEN) 磷酸化蛋白激酶B[p-Akt1(Ser473)] α-平滑肌肌动蛋白(α-SMA) E-钙黏素(E-cadherin) 

分 类 号：R114[医药卫生—卫生毒理学]