小檗碱改善AD模型小鼠认知障碍作用  

Berberine attenuates cognitive deficits via improving NGF signaling in AD mice

作　　者：陈魁敏[1] 周恒伟[2] 李玉芳[1] 金戈[1] 唐迪莹 李欣欣[1] 邢爱平[1] 孟宪锋[3] 

机构地区：[1]沈阳医学院心理教研室,辽宁沈阳110034 [2]沈阳市苏家屯区中心医院 [3]辽宁省精神卫生中心

出　　处：《中国公共卫生》2015年第5期614-616,共3页Chinese Journal of Public Health

基　　金：辽宁省教育厅一般项目(L2014409);沈阳市科技局(F13-220-9-27)

摘　　要：目的观察小檗碱对阿尔茨海默病(AD)模型小鼠脑内神经生长因子(NGF)通路变化及改善AD小鼠学习记忆机制。方法以APP/PS1双转基因小鼠为模型,采用Y迷宫进行学习记忆能力评价,Western blot检测NGF及相关通路蛋白表达。结果与对照组[(77.57±11.81)%]比较,模型组小鼠自发交替反应率[(55.71±12.33)%]降低(P<0.01);与模型组比较,小檗碱组小鼠自发交替反应率[(70.93±15.99)%]升高(P<0.01);与对照组比较,模型组小鼠海马NGF、络氨酸激酶(Trk A)、磷酸化蛋白激酶(ERK)、磷酸化环磷腺苷效应元件结合蛋白(CREB)表达水平[分别为(0.186±0.031)、(0.187±0.019)、(0.572±0.101)、(0.499±0.138)]均降低(均P<0.05);与模型组比较,小檗碱组小鼠海马NGF、Trk A、磷酸化ERK、磷酸化CREB表达水平[分别为(0.592±0.043)、(0.281±0.085)、(0.906±0.097)、(0.895±0.152)]均升高(均P<0.05)。结论小檗碱可以改善APP/PS1小鼠学习记忆障碍,其机制可能与调控NGF通路有关。Objective To investigate the change of nerve grow th factor（ NGF） signaling and mechanisms of berberine on learning and memory deficits in Alzheimer＇s disease（ AD） mice. Methods We adopted APP / PS1 mice as AD animal model; w e used Y maze to measure learning and memory function and Western blot to investigate the protein expression levels. Results Compared with the control mice（ 77. 57 ± 11. 81）,the APP / PS1 mice exhibited reduced spontaneous alternation behavior（ 55. 71 ± 12. 33）（ P〈0. 01）. Berberine（ 30 mg / kg） attenuate the impairment of spontaneous alternation behavior（ 70. 93 ± 15. 99）（ P〈0. 01）. The expression levels of NGF,tyrosine kinases（ Trk A）,phosphorylated extracellular regulated protein kinases（ p-ERK）,and phosphorylated c AM P-response element binding protein（ p-CREB）in the hippocampus were lower in APP / PS1 mice compared to those of the control mice（ P〈0. 05）,but were higher in the berberine（ 30 mg / kg） treated mice compared to those of the APP / PS1 mice（ P〈0. 05）. Conclusion Berberine attenuates learning and memory impairments in APP / PS1 mice,possibly through the NGF signaling pathway.

关 键 词：小檗碱 阿尔茨海默病(AD) 认知障碍 神经生长因子(NGF) 

分 类 号：R114[医药卫生—卫生毒理学]