天然二倍体和四倍体泥鳅鳍细胞系染色体组构成研究  

Study on genosome constitution of natural diploid and tetraploid loach Misgurnus anguillicaudatus fin cells line

作　　者：李雅娟[1] 隋燚 赵睿 李霞[1] 周贺[1] 刘博[1] 马海艳[1] 

机构地区：[1]大连海洋大学辽宁省海洋生物资源恢复及生境修复重点实验室,辽宁大连116023 [2]北京市水产技术推广站,北京101105

出　　处：《东北农业大学学报》2015年第4期83-88,共6页Journal of Northeast Agricultural University

基　　金：国家自然科学基金(31272650);大连海洋大学博士启动基金项目(017208)

摘　　要：对传代20次的天然二倍体和天然四倍体泥鳅鳍细胞系进行染色体标本制备,并对其染色体核型、Ag-NORs及CMA3/DA/DAPI三重荧光染色等进行研究。结果显示,1二倍体泥鳅鳍组织培养细胞的染色体数目为2n=50,核型公式为:2n=10m+4sm+36t,NF=64;四倍体泥鳅鳍组织培养细胞的染色体数目为4n=100,核型公式为:4n=20m+8sm+72t,NF=128。2天然二倍体泥鳅鳍培养的细胞中期染色体分裂相有2个Ag-NORs,天然四倍体泥鳅鳍培养的细胞中期染色体分裂相有4个Ag-NORs,均位于第1对中部着丝粒染色体(M1)的末端。3天然二倍体泥鳅鳍培养的细胞染色体中期分裂相中CMA3阳性位点为2个,天然四倍体泥鳅鳍培养的细胞染色体中期分裂相中CMA3阳性位点为4个;均位于核仁组织区,即第1对中部着丝粒染色体(M1)的末端。结果表明,天然二倍体和四倍体泥鳅鳍培养细胞制备的染色体数目、核型和带型与体细胞一致。Fin cel s of natural diploid and tetraploid loaches subcultured after 20 generations were used for chromosome preparation, and karyotype, Ag-NORs, and CMA3/DA/DAPI were studied primarily. Results showed as follows：①Chromosome number of diploid cells was 2n=50, karyotype was 2n=10m＋4sm＋36t, NF=64;and chromosome number of tetraploid was 4n=100, karyotype was 4n=20m＋8sm＋72t. NF=128.②There were 2 Ag-NORs in the medium chromosome spread of diploid, and 4 in tetraploid, all in the terminal of the first metacentric chromosome（M1）.③Positive CMA3 sites in diploid were 2, and 4 in tetraploid, all in the neuclear organizer region, as the terminal of the first metacentric chromosome（M1）. In conclusion, chromosome number, karyotype and band type of cultured cells of both diploid and tetraploid were consistent with somatic cells. Therefore, methods in this study could be of great value for researches of cytogenetic and molecular genetics of loaches.

关 键 词：泥鳅 二倍体 四倍体 细胞系 染色体组 AG-NORS CMA3/DA/DAPI 

分 类 号：S917.4[农业科学—水产科学]