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作 者:何慧怡[1] 樊丽娜[1] 齐永文[1] 劳方业[1] 邓海华[1]
机构地区:[1]广州甘蔗糖业研究所/广东省甘蔗改良与生物炼制重点实验室,广东广州510316
出 处:《广东农业科学》2015年第5期49-52,I0001,共5页Guangdong Agricultural Sciences
基 金:广东省科技计划项目(2012A090300015;2012B020301008;2012A061400005)
摘 要:以甘蔗品种新台糖22和粤糖93-159的愈伤组织和刚分化4周的小苗为试验材料,初步确定了灭草烟在愈伤组织分化阶段和刚分化4周小苗的筛选浓度。结果发现,不同基因型甘蔗的愈伤组织和刚分化小苗对灭草烟的耐受浓度均有差异。综合试验结果,适合灭草烟在新台糖22和粤糖93-159愈伤组织分化阶段的筛选浓度为0.3μmol/L;适合灭草烟对新台糖22刚分化4周小苗的筛选浓度为0.5μmol/L,适合灭草烟对粤糖93-159刚分化4周小苗的筛选浓度为1.0μmol/L。With the callus differentiation and shoot for 4 weeks of sugarcane variety ROC 22 and Yuetang 93-159 as materials, this paper determined the concentration of imazapyr in callus differentiation stage and shoot stage of the two sugarcane varieties. The results showed that the callus tissue and shoot for 4 weeks of different genotypes of sugarcane had different tolerance to imazapyr. At the callus differentiation stage, the suitable imazapyr concentration for ROC 22 and Yuetang 93-159 was 0.3 μmol/L. At the shoot stage of differentiation for 4 weeks, the suitable imazapyr concentration for ROC 22 was 0.5 μmol/L, while was 1.0 μmol/L for Yuetang 93-159.
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