子痫前期孕妇孕早期血清miR-520g表达分析  

The analysis of serum miR-520g expression in the early stage of preeclampsia among pregnant patients

作　　者：厉倩[1] 龙安雄[1] 洪茂[1] 蔡雷鸣[1] 谢骊[1] 顾继安[1] 谭龙益[1] 

机构地区：[1]上海市第一人民医院宝山分院检验科,上海200940

出　　处：《检验医学》2015年第4期345-348,共4页Laboratory Medicine

基　　金：上海市卫生局青年科研项目(20124Y080)

摘　　要：目的探讨血清微小RNA(miR)-520g在子痫前期(PE)患者孕期的变化及意义。方法分别提取30例PE患者和30名正常妊娠者早、中、晚孕期血清中的循环miR,利用实时荧光定量逆转录聚合酶链反应(PCR)检测血清中的miR-520g的表达量,比较两组间不同孕期miR-520g的差异。结果正常妊娠组随孕程进展血清miR-520g的表达量增加;与正常妊娠组相比,PE组孕早期miR-520g显著增加,PE组孕中、晚期的血清miR-520g表达量与正常妊娠组差异无统计学意义。结论孕早期血清miR-520g可能是PE预测的良好标志物。Objective To investigate the change and significance of serum microRNA （ miR）-520g in preeclampsia among pregnant patients .Methods Serum cycling miR was extracted from 30 patients with preeclampsia and 30 healthy pregnant women at early , middle and late pregnant stages .The expression of miR-520g was determined by quantitive reverse transcription polymerase chain reaction （ PCR ） , and the difference between the 2 groups was analyzed statistically.Results MiR-520g increased with pregnant days in healthy pregnant group .MiR-520g in early pregnant stage of preeclampsia group increased comparing with that of healthy pregnant group , whereas no significant difference was observed in middle and late pregnant stages between preeclampsia group and healthy pregnant group .Conclusions Serum miR-520g in early pregnant stage may be a potential biomarker for the prediction of preeclampsia .

关 键 词：子痫前期 微小RNA 血清 生物标志物 

分 类 号：Q503[生物学—生物化学]